Optimization of competitively differentiated polymerase chain reaction in detection of HBV basal core promoter mutation

Table 1 Primers and probe.

Denomination	Sequences
PCSc	5’-CCCGAATTCCACCGTGAACGCCCATCAG-3’
PCAc	5’-CCCAAGCTTGCAGTATGGTGAGGTGAGCAATG-3’
BCP-c1	5’-CGGAATTCGAGGAGCTGGGGGAGGAGATTAGGTTAATGATC-3’
BCP-c2	5’-AGACTGCAGAGGATAGAATCTAGCAGGC-3’
BCP1-W	5’-FITC-CGTCCGTAGTGCCGAGGGAGGAGATTAGGTTAAAGG-3’
BCP1-M	5’-DIG -CGCTGAGATGCGACTGGGAGGAGATTAGGTTAATGA-3’
PCA	5’-CTCGCTGCACACTCTAAGGCTTCTCGATACAG-3’
PCP	5’-BIO-GACGGAAGGAAAGAAGTCAGAAGGCAAAA-3’

The underlined nucleotides were loci of restriction endonucleases. The italic characters had no relationship with HBV genome.

Table 2 A1762T/G1764A mutant detection using optimized CD-PCR in clinical sera.

	Cases(n)	HBV DNA n (%)	A1762T/G1764A n (%)
HBsAg(+)/HBeAg(+)	60	60 (100.0)	19 (31.7)
HBsAg(+)/HBeAg(-)	100	62 (62.0)1	32 (51.6)2
HBsAg(-)/HBeAg(-)	40	0	0

¹χ² = 29.90, P<0.01,

²χ² = 4.99, P<0.05, compared with the group of HBsAg (+)/HBeAg (+).