Copyright
©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Jun 21, 2005; 11(23): 3523-3527
Published online Jun 21, 2005. doi: 10.3748/wjg.v11.i23.3523
Published online Jun 21, 2005. doi: 10.3748/wjg.v11.i23.3523
Table 1 PCR primers for amplification of cagA, vacA and iceA sequences.
Gene and DNA region | Prime | Primer sequence (5’-3’) | Size of PCR product (bp) | References |
cagA | CAGAF | GGCAATGGTGGTCCTGGAGCTAGGC | 324 | Pan[21] |
CAGAR | GGAAATCTTTAATCTCAGTTCGG | |||
vacAs1 and s2 | VA1-F | ATGGAAATACAACAAACACAC | 259/286 | Atherton[22] |
VA1-R | CTGCTTGAATGCGCCAAAC | |||
m1a | VA3-F | GGTCAAAATGCGGTCATGG | 290 | Atherton[22] |
VA3-R | CCATTGGTACCTGTAGAAAC | |||
m1b | VAm-F3 | GGCCCCAATGCAGTCATGGAT | 291 | Atherton[23] |
VAm-R3 | GCTGTTAGTGCCTAAAGAAGCAT | |||
m2 | VA4-F | GGAGCCCCAGGAAACATTG | 352 | Atherton[22] |
VA4-R | CATAACTAGCGCCTTGCAC | |||
iceA1 | iceA1F | GTGTTTTTAACCAAAGTATC | 247 | Peek[10] |
iceA1R | CTATAGCCASTYTCTTTGCA | |||
iceA2 | iceA2F | GTTGGGTATATCACAATTTAT | 229/334 | Peek[10] |
iceA2R | TTRCCCTATTTTCTAGTAGGT |
Table 2 Distribution of cagA,vacA and iceA genotypes in Zhanghe and Shenyang.
Gene | Zhuanghe area (%) n = 107 | Shenyang area (%) n = 42 |
cagA+ | 101 (94.4) | 42 (100.00) |
cagA- | 6 (5.6) | 0 (0) |
vacA s1 | 102 (95.33)b | 27 (64.29) |
vacA s2 | 0 (0) | 0 (0) |
vacA s- | 5 (4.67)d | 15 (35.71) |
vacA m1a | 0 (0) | 0 (0) |
vacA m1b | 24 (22.43) | 5 (11.9) |
vacA m2 | 26 (24.3) | 11 (26.19) |
vacA m1b/m2 | 48 (44.96) | 22 (52.38) |
vacA m- | 9 (8.41) | 4 (9.52) |
iceA1 | 10 (9.35) | 1 (2.38) |
iceA2 | 10 (9.35) | 1 (2.38) |
iceA1/iceA | 2 84 (78.5) | 32 (76.19) |
iceA- | 3 (2.8) | 8 (19.05) |
Table 3 vacA genotypes of H pylori strains in Zhanghe and Shenyang.
Table 4 Combination genotypes of cagA,vacA and iceA in Zhuanghe and Shenyang, n (%).
cagA | vacAs | vacAm | iceA | Zhuanghe (n = 107) | Shenyang (n = 42) |
cagA | s1 | m1b | A1 | 3 (2.8) | 0 (0) |
cagA | s1 | m1b | A2 | 3 (2.8) | 0 (0) |
cagA | s1 | m1b | A1+A2 | 13 (12.15) | 0 (0) |
cagA | s1 | m2 | A1 | 3 (2.8) | 0 (0) |
cagA | s1 | m2 | A2 | 1 (0.935) | 1 (2.38) |
cagA | s1 | m2 | A1+A2 | 18 (16.82) | 5 (11.9) |
cagA | s1 | m1b+m2 | A1+A2 | 39 (36.45) | 14 (33.3) |
cagA | s | m1b+m2 | A1+A2 | 2 (1.87) | 7 (16.67) |
Others | 25 (23.36) | 15 (14.02) |
Table 5 Multiple strains infection in Zhuanghe and Shenyang, n (%).
Genotype | Zhuanghe (n = 107) | Shenyang (n = 42) |
vacA m1/m2 | 48 (44.86) | 22 (52.38) |
iceA1/iceA2 | 84 (78.5) | 32 (76.19) |
vacAm1/m2 or iceA1/iceA | 92 (85.98) | 33 (78.57) |
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Citation: Gong YH, Wang Y, Yuan Y. Distribution of
Helicobacter pylori in north China. World J Gastroenterol 2005; 11(23): 3523-3527 - URL: https://www.wjgnet.com/1007-9327/full/v11/i23/3523.htm
- DOI: https://dx.doi.org/10.3748/wjg.v11.i23.3523