Deletion of cagA gene of Helicobacter pylori by PCR products

Table 1 Primers for amplifying Cmr and up- and downstream regions of cagA.

Primer	Sequence (5'-3')	Expected product size
C1	GAT ATA GAT TGA AAA GTG GAT[20]	742
C2	TTA TCA GTG CGA CAA ACT GGG[20]
P1	GCC ACT ACT ACC ACC GAC AT	364
P2	ATC CAC TTT TCA ATC TAT ATC TAT GAC T AA GCC ACT GCC GT
P3	CCC AGT TTG TCG CAC TGA TAA TCA AAT ACA CCA ACG CCT	484
P4	GCA TCC CTA TTA GCC TCT T
B1	ATT AGA CAA CTT GAG CGA G	276
B2	ACA AAC ATC ACG CCA TC
E1	ATG CGA GCC TAT AAT GAG AAG C	841
E2	GAA GCG TGA TAA AAG AGC AAT GTT

Lineated sequences are complementary to C1 and C2. E1, E2 are positive controls for cagE gene.