Basic Research
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Apr 28, 2005; 11(16): 2431-2437
Published online Apr 28, 2005. doi: 10.3748/wjg.v11.i16.2431
Table 1 Influence of different concentrations of NCTD on Matrigel invasion of GBC-SD cells.
GroupNCTD (mg/mL)nPassing-membrane cells (% of control group)Dead passing-membrane cells (% of control group)
Control06100.017.2±1.15
NCTD5662.0±5.08b31.7±4.29b
10637.7±2.01b59.7±1.15b
20618.0±1.79b67.3±4.93b
4065.0±1.80b95.2±4.88b
Table 2 Influence of different concentration of NCTD on the crossing-river time of GBC-SD cells.
GroupNCTD (mg/mL)nCrossing-rivertime (h)
Control0622.6±0.67
NCTD5628.2±1.00b
10633.4±0.71b
20645.0±0.68b
30658.8±0.72b
406>72b
Table 3 Influence of NCTD on expression of PCNA, Ki-67 proteins of GBC-SD cells.
GroupnPositive index (mean±SD)
PCNAKi-67
Control100.932±0.0310.964±0.092
IC50 NCTD (56.18 mg/mL)100.318±0.023a0.297±0.018a
Table 4 Influence of NCTD on expression of MMP2, TIMP2 proteins and the MMP2 to TIMP2 ratio of GBC-SD cells.
GroupNCTD (mg/mL)nPercent of protein expression (%)
MMP2/TIMP2
MMP2TIMP2
Control01062.82±3.2013.42±1.274.68
NCTD51034.61±1.82a30.79±2.15a1.12b
101027.02±1.40a34.22±2.97a0.79b
201026.18±1.81a35.60±2.04a0.74b
401019.56±2.36 a29.54±2.33a0.66b