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©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Jan 7, 2005; 11(1): 31-35
Published online Jan 7, 2005. doi: 10.3748/wjg.v11.i1.31
Published online Jan 7, 2005. doi: 10.3748/wjg.v11.i1.31
Table 1 Primers for DNA sequencing and AS-PCR.
| Primer | Forward primer | Position | Reverse primer |
| P1 | TAGACGGGCTCACATCAC | 620 | GGGGTATCTAATAGTTTGGGT |
| P2 | GCAAGCATCCCCGTTCCAGT | 697 | GGGGTATCTAATAGTTTGGGT |
| P3 | GCAAGCATCCCCGTTCCAGG | 697 | GGGGTATCTAATAGTTTGGGT |
Table 2 Variations of mitochondrial 12S rRNA in gastric carcinoma.
| Position (np) | Variations | Property |
| 652 | G-ins1 | HM |
| 663 | A-G | PM |
| 709 | G-A | PM |
| 716 | T-G1 | HM |
| 728 | C-T | PM |
| 745 | A-G1 | PM |
| 750 | G-A | PM |
| 757 | A-T | PM |
| 759 | A-T1 | PM |
| 764 | A-C | PM |
| 772 | A-T1 | PM |
| 782 | A-T1 | PM |
| 794 | T-C1 | PM |
| 799 | A-C1 | PM |
Table 3 Comparison of content of mutant-type 12S rRNA in gastric cancerous cells and dysplasia cells.
| Num. | Cancer (T3) | Dysplasia (D3) |
| 1 | 0.9214 | 0.8164 |
| 2 | 0.8738 | 0.6487 |
| 3 | 0.9038 | 0.7268 |
| 4 | 0.8955 | 0.5572 |
| 5 | 0.8659 | 0.6402 |
| Mean±D | 0.89±0.02 | 0.68±0.09 |
| P | <0.01 |
- Citation: Han CB, Ma JM, Xin Y, Mao XY, Zhao YJ, Wu DY, Zhang SM, Zhang YK. Mutations of mitochondrial 12S rRNA in gastric carcinoma and their significance. World J Gastroenterol 2005; 11(1): 31-35
- URL: https://www.wjgnet.com/1007-9327/full/v11/i1/31.htm
- DOI: https://dx.doi.org/10.3748/wjg.v11.i1.31