Amino acid uptake in arterio-venous serum of normal and cancerous colon tissues

Table 1 A-V amino acid uptake rates in normal and cancerous colon tissue

Amino acid	Sample number	R2 (%)	R1 (%)	P value
Threonine 13	16	13.50 ± 3.76	39.25 ± 7.59	< 0.01
Phenylalanine 13	16	6.37 ± 1.77	26.26 ± 4.31	< 0.01
Leucine 14	16	8.21 ± 2.13	36.55 ± 6.11	< 0.01
Isoleucine 13	15	11.39 ± 3.55	30.63 ± 5.50	< 0.01
Methionine 1	16	-0.13 ± 1.20	11.55 ± 5.22	> 0.05
Valine 1	16	7.67 ± 2.42	33.46 ± 5.56	< 0.01
Lysine 14	16	-8.01 ± 1.85	25.44 ± 4.41	< 0.01
Histidine 1	16	1.06 ± 0.94	29.83 ± 6.37	< 0.01
Proline 2	11	-26.86 ± 8.36	6.49 ± 1.69	< 0.02
Glutamine 2	16	-12.42 ± 3.12	18.70 ± 5.23	< 0.01
Ammonia 2	16	-23.61 ± 4.58	4.70 ± 1.97	< 0.05
Alanine 2	16	-9.65 ± 2.47	18.16 ± 2.29	< 0.01
Glycine 2	16	-1.50 ± 0.98	29.18 ± 4.41	< 0.01
Cystine 2	13	-10.01 ± 3.37	8.89 ± 1.58	< 0.01
Arginine 2	16	-1.74 ± 0.88	29.02 ± 4.93	< 0.01
Serine 2	16	2.39 ± 1.02	37.26 ± 5.67	< 0.01
Tyrosine 23	16	6.57 ± 1.45	26.33 ± 3.39	< 0.01

¹Refers to essential amino acids,

²refers to non-essential amino acids,

³refers to ketogenic and glucogenic amino acids,

⁴refers to ketogenic amino acids.

Table 2 Relationship between A-V amino acid uptake rates and tumor size in colon cancer

Amino acid	Tumor size < 5.0 cm		Tumor size > 5.0 cm		P value
	Sample number	R1 (%)	Sample number	R1 (%)
Threonine	7	35.25 ± 10.13	9	42.37 ± 7.81	0.58
Phenylalanine	7	25.44 ± 10.63	9	26.89 ± 3.81	0.889
Leucine	7	33.02 ± 12.85	9	39.30 ± 6.53	0.648
Isoleucine	6	30.09 ± 16.48	9	30.99 ± 4.34	0.95
Methionine	7	13.53 ± 7.47	9	9.99 ± 8.79	0.772
Valine	7	27.63 ± 8.37	9	37.99 ± 8.50	0.408
Lysine	7	21.17 ± 14.07	9	28.76 ± 7.36	0.618
Histidine	7	25.41 ± 10.03	9	33.26 ± 7.26	0.528
Proline	4	10.05 ± 9.81	7	4.45 ± 7.19	0.653
Glutamine	7	25.45 ± 8.69	9	13.44 ± 11.93	0.455
Ammonia	7	13.92 ± 9.08	9	2.47 ± 7.08	0.179
Alanine	7	14.97 ± 1.56	9	20.64 ± 7.72	0.679
Glycine	7	30.04 ± 1.14	9	28.52 ± 11.24	0.926
Cystine	7	20.82 ± 0.69	8	7.23 ± 10.54	0.329
Arginine	7	21.68 ± 7.09	9	34.73 ± 9.06	0.485
Serine	7	25.40 ± 3.22	9	38.71 ± 9.36	0.836
Tyrosine	7	24.90 ± 0.66	9	27.44 ± 4.56	0.815

Table 3 Relationship between A-V amino acid uptake rates and Dukes stage in colon cancer

Amino acid	Dukes stage B		Dukes stage C or D		P value
	Sample number	R1 (%)	Sample number	R1 (%)
Threonine	10	35.75 ± 8.85	6	45.10 ± 7.10	0.476
Phenylalanine	10	24.26 ± 7.02	6	29.60 ± 6.47	0.616
Leucine	10	29.73 ± 9.68	6	47.93 ± 4.07	0.184
Isoleucine	9	22.74 ± 10.23	6	42.47 ± 4.41	0.158
Methionine	10	2.44 ± 6.39	6	26.72 ± 8.30	0.036
Valine	10	34.85 ± 9.53	6	31.15 ± 3.37	0.776
Lysine	10	20.10 ± 11.03	6	34.34 ± 4.90	0.356
Histidine	10	25.32 ± 8.83	6	37.35 ± 5.08	0.341
Proline	6	-2.70 ± 6.43	5	17.50 ± 7.25	0.066
Glutamine	10	26.30 ± 9.92	6	6.02 ± 10.95	0.209
Ammonia	10	5.44 ± 7.74	6	3.47 ± 10.07	0.879
Alanine	10	21.57 ± 9.65	6	12.50 ± 6.66	0.516
Glycine	10	29.37 ± 8.65	6	28.87 ± 15.91	0.976
Cystine	9	11.46 ± 10.67	6	16.73 ± 6.03	0.715
Arginine	10	23.12 ± 13.84	6	38.85 ± 4.80	0.409
Serine	10	34.32 ± 9.75	6	42.16 ± 12.82	0.632
Tyrosine	10	23.08 ± 7.78	6	31.74 ± 4.30	0.432