In vitro effects of recombinant human growth hormone on growth of human gastric cancer cell line BGC823 cells

doi:10.3748/wjg.v10.i8.1132

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 10, Issue 8

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (2521)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-7) series, Tables (1-6) series.

Item

Count

PDF

254

HTML

1862

Figures (1-7)

200

Tables (1-6)

205

Sum=2521

Apr 15, 2004 (publication date) through Nov 26, 2024

Times Cited of This Article

Times Cited (9)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Gastric Cancer

World J Gastroenterol. Apr 15, 2004; 10(8): 1132-1136
Published online Apr 15, 2004. doi: 10.3748/wjg.v10.i8.1132

Table 1 Experimental groups and concentrations of drugs

Groups	Names and concentrations of drugs
I	RPMI1640
II	L-OHP 4 µg/mL
III_a	rhGH 50 ng/mL
III_b	rhGH 100 ng/mL
III_c	rhGH 200 ng/mL
III_d	rhGH 400 ng/mL
IV_a	rhGH 50 ng/mL + L-OHP 4 µg/mL
IV_b	rhGH 100 ng/mL + L-OHP 4 µg/mL
IV_c	rhGH 200 ng/mL + L-OHP 4 µg/mL
IV_d	rhGH 400 ng/mL + L-OHP 4 µg/mL

Table 2 Scheme of drugs in each group

Group	I	II	III_a	III_b	III_c	III_d	IV_a	IV_b	IV_c	IV_d
L-OHP (µL)	/	50	/	/	/	/	50.0	50	50	50
rhGH (µL)	/	/	12.5	25	50	100	12.5	25	50	100
RPMI1640 (µL)	150	100	137.5	125	100	50	87.5	75	50	/

Table 3 Effects of rhGH and L-OHP on BGC823 cells (n=4, mean ± SD)

Groups	OD values	Survival rate (%)	Inhibitory rate (%)
I	0.863 ± 0.172	100.00	0
II	0.425 ± 0.086^b	49.25^b	50.75^b
III_a	0.947 ± 0.142	109.73	-9.73
III_b	0.894 ± 0.220	103.59	-3.59
III_c	0.848 ± 0.346	98.26	1.74
III_d	0.844 ± 0.196	97.79	2.21
IV_a	0.338 ± 0.240^b	39.17^b	60.83^b
IV_b	0.318 ± 0.038^b	36.85^b	63.15^b
IV_c	0.318 ± 0.018^b	36.85^b	63.15^b
IV_d	0.306 ± 0.200^b	35.46^b	64.54^b

^bP<0.01 vs control group or rhGH group.

Table 4 Percentages of BGC823 cells in various phases of cell cycle (n = 3, mean ± SD)

Group	G₀–G₁(%)	S (%)	G₂–M(%)
I	47.75 ± 0.78	36.95 ± 0.49	15.25 ± 0.21
II	83.85 ± 1.77^b	7.85 ± 0.64^b	8.30 ± 1.13^b
III_a	53.03 ± 4.31	32.17 ± 7.47	14.73 ± 3.40
III_b	52.33 ± 4.55	32.63 ± 6.19	14.73 ± 1.88
III_c	52.33 ± 4.94	33.50 ± 8.17	14.17 ± 3.29
III_d	51.27 ± 5.94	33.67 ± 8.89	15.07 ± 3.07
IV_a	85.07 ± 2.62^b	6.67 ± 3.88^b	8.00 ± 1.82^b
IV_b	86.47 ± 2.07^b	5.47 ± 1.88^b	7.77 ± 0.40^b
IV_c	85.53 ± 0.47^b	7.87 ± 4.99^b	6.37 ± 5.61^b
IV_d	86.13 ± 2.87^b	4.20 ± 1.61^b	9.67 ± 1.27^b

^bP<0.01 vs control group or rhGH group.

Table 5 Proliferation index in each group (n = 3, mean ± SD)

Group	PI
I	52.25 ± 0.78
II	16.15 ± 1.77^b
III_a	46.97 ± 4.31
III_b	47.67 ± 4.55
III_c	47.67 ± 4.94
III_d	48.73 ± 5.94
IV_a	14.93 ± 2.62^b
IV_b	13.53 ± 2.07^b
IV_c	14.47 ± 0.47^b
IV_d	13.87 ± 2.87^b

^bP<0.01 vs control group or rhGH group.

Table 6 DNA inhibitory rate in rhGH group and rhGH+L-OHP group (n = 3, mean ± SD)

Group	DNA inhibitory rate (%)
III_a	111.1 ± 9.4
IV_a	178.1 ± 6.8
III_b	109.5 ± 9.8
IV_b	181.0 ± 4.0
III_c	109.5 ± 10.5
IV_c	178.2 ± 1.9
III_d	107.2 ± 12.4
IV_d	180.3 ± 7.6

Citation: Chen JY, Liang DM, Gan P, Zhang Y, Lin J. In vitro effects of recombinant human growth hormone on growth of human gastric cancer cell line BGC823 cells. World J Gastroenterol 2004; 10(8): 1132-1136
URL: https://www.wjgnet.com/1007-9327/full/v10/i8/1132.htm
DOI: https://dx.doi.org/10.3748/wjg.v10.i8.1132