Expression of Egr-1, c-fos and cyclin D1 in esophageal cancer and its precursors: An immunohistochemical and in situ hybridization study

Table 1 Expression of Egr-1, c-fos and cyclin D1 mRNAs and their proteins in esophageal precancerous lesions and cancer tissues

Groups	n	Egr-1 positivity		c-fos positivity		cyclin D1 positivity
		ISH(%)	IHC (%)	ISH(%)	IHC(%)	ISH(%)	IHC(%)
Normal epithelia	42	13 (31.0)	3 (7.1)	2 (4.8)	1 (2.4)	2 (4.8)	0 (0.0)
Simple hyperplasia	54	18 (33.3)	8 (14.8)	3 (5.6)	3 (5.6)	5 (9.3)	3 (5.6)
Dysplasia	44	27 (61.4)	21 (47.7)	30 (68.2)	29 (65.9)	6 (13.6)	6 (13.6)
Carcinoma in situ	2	1 (50.0)	1 (50.0)	1 (50.0)	1 (50.0)	0 (0.0)	0 (0.0)
Invasive carcinoma	68	12 (17.6)	9 (13.2)	35 (51.5)	36 (52.9)	35 (51.5)	33 (48.5)

^bP < 0.01, χ² = 16.206, compared with dysplasia group, ^dP < 0.01, χ² = 16.367, compared with dysplasia group.

Table 2 Association of expression of c-fos and cyclin D1mRNAs and their proteins with differentiation degree of cancer tissues

Groups	n	c-fos expression								cyclin D1 expression
		ISH				IHC				ISH				IHC
		-	+	++	+++	-	+	++	+++	-	+	++	+++	-	+	++	+++
Squamous carcinoma
Grade I	23	10	4	5	4	6	4	4	9	13	6	4	0	13	4	5	1
Grade II	33	14	7	8	4	16	3	5	9	17	7	6	3	19	6	5	3
Grade III	12	9	0	2	1	10	1	0	1	3	0	3	6	3	1	2	6b

^bP < 0.01, χ² = 11.256, compared with squamous cell carcinoma grades I and II.

Table 3 Association of expression of c-fos and cyclin D1 with lymphic metastasis in esophageal carcinomas

Groups	n	c-fos positivity		cyclin D1 positivity
		ISH	IHC	ISH	IHC
Lymphatic metastasis
Negative	42	23	20	21	20
Positive	23	12	16	14	13

^aP > 0.05, χ² = 0.767, compared with lymphatic metastasis negative group; ^cP > 0.05, χ² = 0.003, compared with lymphatic metastasis negative group.