Hemizygous deletion and hypermethylation of RUNX3 gene in hepatocellular carcinoma

Table 1 Primers used for PCR amplification of RUNX3 gene

Name	Exon	Primer sequence	Annealing Tm(°C)	Size (bp)
RUNX3_E1	1	F:5’-CTGGCCACAGCTCCCCACC-3’	64	158
		R:5’-ATCCCAACCCAACCCCCTGAAG-3’
RUNX3_E2-1	2	F5’-CTGCTTTCCCGCTTCTCGCGGCAGC-3’	62	212
		R:5’-CGCCGCTGTTCTCGCCCATCTTGCC-3’
RUNX3_E2-2	2	F:5’-CCAAGCACCAGCCGCCGCTTCACAC-3’	65	207
		R:5’-GAGGAAGTTGGGGCTGTCGGTGCGC-3’
RUNX3_E2-3	2	F:5’-CTCGATGGTGGACGTGCTGGCGGAC-3’	62	230
		R:5’-GTCTCGGGCACCTCCCATCCCCACT-3’
RUNX3_E3	3	F:5’-TGCCATTGCCAATGCTGAA-3’	58	240
		R:5’-TAAGCTGTCCCCCTGCATCC-3’
RUNX3_E4	4	F:5’-GCACTGGACCCTCCTCCC-3’	60	187
		R:5’-CACCTGCCTCTATTCCCCACT-3’
RUNX3_E5	5	F:5’-CGCTGCAGCCCCTCCCTC-3’	65	225
		R:5’-GGGGAAGCAACGGCTGATGG-3’
RUNX3_E6-1	6	F:5’-GTTGTTAGGGTCCCCGCCTCC-3’	63	224
		R:5’-CTACCCGCATGCTGGCCTCTT-3’
RUNX3_E6-2	6	F:5’-CGCTGCTGGTGCAAGAGG-3’	63	205
		R:5’-ACCAGCCGCTTCCACCATAC-3’
RUNX3_E6-3	6	F:5’-TGGCGGGAGGTAGGTATGGTG-3’	63	166
		R:5’-GCCAACCCTCACGGAGAGC-3’
RUNX3_E6-4	6	F:5’-AATGCATCCTGGGGTCTGG-3’	61	150
		R:5’-GGGTTGTCTCGAGCTGGAACT-3’