Copyright
©The Author(s) 2004.
World J Gastroenterol. Feb 1, 2004; 10(3): 366-370
Published online Feb 1, 2004. doi: 10.3748/wjg.v10.i3.366
Published online Feb 1, 2004. doi: 10.3748/wjg.v10.i3.366
Table 1 Protocol of TRAP reaction
| Step | Time | Temperature | Cycle |
| number | |||
| Primer elongation | 30 min | 25 | 1 |
| Telomerase inactivation | 5 min | 94 | 2 |
| Amplification | 3 - 32 | ||
| Denaturation | 30 s | 94 | |
| Annealing | 30 s | 50 | |
| Polymerization | 90 s | 72 | |
| 10 min | 72 | 33 |
- Citation: Liu SX, Sun WS, Cao YL, Ma CH, Han LH, Zhang LN, Wang ZG, Zhu FL. Antisense oligonucleotide targeting at the initiator of hTERT arrests growth of hepatoma cells. World J Gastroenterol 2004; 10(3): 366-370
- URL: https://www.wjgnet.com/1007-9327/full/v10/i3/366.htm
- DOI: https://dx.doi.org/10.3748/wjg.v10.i3.366