Nude mice model of human hepatocellular carcinoma via orthotopic implantation of histologically intact tissue

Table 1 Primers for MDR1 and LRP

Gene	Size (bp)	Quantification method	Sequence (5’-3’)
B-actin	530	Forward primer	GTGCGTGACATTAAGGAG
		Reverse primer	CTAAGTCATAGTCCGCCT
MDR1	174	Forward primer	CATTGGTGTGGTGAGTCAGG
		Reverse primer	CTCTCTCTCCAACCAGGGTG
LRP	237	Forward primer	TAAGGGCTTCCAGCACCAAC
		Reverse primer	GGAGTTCTCGCTTCTCGTCC

Table 2 Expression of MDR1 and LRP in HCC from donor and nude mice

	Immunohistochemistry (%)		mRNA of RT-PCR (%)
	MDR1	LRP	Mdr1	LRP
Donor	31.26	27.18	61.47	56.71
Nude mice	29.20 ± 2.81	25.44 ± 2.43	65.42 ± 13.59	58.63 ± 9.37
t	2.077	2.030	0.823	2.288
P	0.076	0.080	0.438	0.056