Expression of liver insulin-like growth factor 1 gene and its serum level in rats with diabetes

Table 1 Glucose metabolic parameters during the experiment

Group	Duration (month)	n	Initial weight (g)	Weight (g)	Blood (mmol·L-1)	Aminofructose (mmol·L-1)
NC	0.5	5	198.41 ± 9.76	249.33 ± 16.02	5.14 ± 0.91	0.82 ± 0.07
	2	5	202.53 ± 17.67	351.20 ± 18.23	5.3 ± 0.44	0.85 ± 0.08
	3	6	200.06 ± 13.03	402.05 ± 37.10	4.91 ± 0.26	0.81 ± 0.09
	6	5	199.65 ± 15.22	544.54 ± 30.41	5.21 ± 0.47	0.85 ± 0.05
ID-1	0.5	5	196.25 ± 14.22	254.31 ± 20.97	4.93 ± 0.72	0.79 ± 0.05
	2	4	202.34 ± 19.12	345.75 ± 23.48	5.10 ± 0.62	0.84 ± 0.08
	3	5	196.42 ± 11.41	411.31 ± 47.37	4.88 ± 0.67	0.78 ± 0.06
	6	6	198.68 ± 12.64	538.52 ± 31.62	4.94 ± 0.58	0.84 ± 0.07
ID-2	0.5	5	192.00 ± 5.70	217.00 ± 9.64a	11.4 ± 0.56c	1.02 ± 0.14c
	2	5	199.00 ± 16.73	241.00 ± 16.44c	10.94 ± 1.08c	1.00 ± 0.29c
	3	6	198.66 ± 14.36	256.83 ± 14.98c	10.86 ± 0.94c	0.98 ± 0.08c
	6	5	201.37 ± 14.11	266.24 ± 13.53c	12.13 ± 0.63c	1.10 ± 0.14c
ID-3	0.5	5	208.60 ± 13.08	205.75 ± 15.34b	18.34 ± 1.03ce	1.20 ± 0.12ce
	2	5	198.60 ± 16.66	192.80 ± 13.35cd	17.48 ± 0.62ce	1.18 ± 0.21ce
	3	5	211.50 ± 11.37	204.8 ± 11.03ce	17.50 ± 1.05ce	1.21 ± 0.19ce
	6	6	204.35 ± 12.34	185.22 ± 14.36ce	16.89 ± 0.95ce	1.2 ± 0.34ce

Data expressed as mean ± SD. NC, normal control group; ID-1,-2 ,-3, insulin treatment group. vs NC,

^aP < 0.0025,

^bP < 0.001,

^cP < 0.0001; vs ID-2 (for the same period),

^dP < 0.001,

^eP < 0.0001.

Table 2 Liver tissue IGF-1mRNA ,peptide contents and IGF-1serum concentration

Group	Duration (month)	n	Liver tissue mRNA contents*	Liver tissue IGF-1 peptide (ng·mg-1)**	Serum IGF-1 (ng·ml-1)
NC	0.5	5	1.15 ± 0.09	196.66 ± 14.9	511.2 ± 24.7
	2	5	1.17 ± 0.069	198.13 ± 15.25	544.6 ± 22.4
	3	6	1.12 ± 0.056	202.05 ± 15.73	525 ± 30.2
	6	5	1.14 ± 0.066	197.11 ± 12.55	520.7 ± 26.32
ID-1	0.5	5	1.20 ± 0.064	196.7 ± 17.4	536 ± 18.1
	2	4	1.21 ± 0.054	204.1 ± 16.5	540.5 ± 32.5
	3	5	1.18 ± 0.047	200.42 ± 14.9	520.2 ± 14.4
	6	6	1.22 ± 0.044	199.38 ± 16.56	536.54 ± 25.14
ID-2	0.5	5	0.79 ± 0.048b	128.2 ± 11.25c	371.0 ± 12.5c
	2	5	0.74 ± 0.028b	121.3 ± 7.27c	366.4 ± 16.0c
	3	6	0.71 ± 0.024bh	114.35 ± 8.09ci	353.5 ± 22.4ce
	6	5	0.68 ± 0.035bh	110.38 ± 10.57ci	349.6 ± 18.62ci
ID-3	0.5	5	0.53 ± 0.023cf	74.43 ± 5.33df	223.2 ± 9.39dc
	2	5	0.49 ± 0.016cf	67.4 ± 6.07df	205.6 ± 12.7dc
	3	5	0.47 ± 0.02dgj	64.58 ± 3.89dgj	196.4 ± 15.67dgj
	6	6	0.44 ± 0.08dgj	62.91 ± 4.32dgj	188.5 ± 17.35dgj

Data expressed as mean ± SD. *IGF-1relative mRNA contents: IGF-1 cDNA/β-actin cDNA, **tissue IGF-1peptide content: IGF-1 ng·mg^-1 total protein. ID-1,-2 ,-3 vs NC (for the same period):

^bP < 0.001,

^cP < 0.0005.

^dP < 0.0001; vs ID-2 group (for the same period):

^eP < 0.025,

^fP < 0.0025,

^gP < 0.001; vs ID-2 (week 2):

^hP < 0.05,

ⁱP < 0.01; vs ID-3 (week 2):

^jP < 0.01.