Basic Research
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jan 15, 2004; 10(2): 255-259
Published online Jan 15, 2004. doi: 10.3748/wjg.v10.i2.255
Expression of liver insulin-like growth factor 1 gene and its serum level in rats with diabetes
Jian-Bo Li, Cheng-Ya Wang, Jia-Wei Chen, Zhen-Qing Feng, Hong-Tai Ma
Jian-Bo Li, Jia-Wei Chen, Department of Endocrinology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
Cheng-Ya Wang, Molecular Laboratory, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
Zhen-Qing Feng, Hong-Tai Ma, Department of Pathology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 39770355
Correspondence to: Dr. Jian-Bo Li, Department of Endocrinology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. ljbzjlx18@yahoo.com.cn
Telephone: +86-25-3718836-6983 Fax: +86-25-3724440
Received: June 4, 2003
Revised: August 9, 2003
Accepted: August 16, 2003
Published online: January 15, 2004
Abstract

AIM: To explore the effect of diabetic duration and blood glucose level on insulin like growth factor 1 (IGF-1) gene expression and serum IGF-1 level.

METHODS: Diabetes was induced into Sprague Dawley rats by alloxan and then the rats were subdivided into different groups with varying blood glucose level and diabetic duration. The parameters were measured as follows: IGF-1 mRNA by reverse transcriptase- polymerase chain reaction (RT-PCR), IGF-1 peptide and serum IGF-1 concentration by enzyme-linked immunosorbent assay (ELISA).

RESULTS: During early diabetic stage (week 2), in comparison with normal control group (NC), IGF-1 mRNA (1.17 ± 0.069 vs 0.79 ± 0.048, P < 0.001; 1.17 ± 0.069 vs 0.53 ± 0.023, P < 0.0005, respectively), IGF-1 peptide contents [(196.66 ± 14.9) ng·mg-1vs (128.2 ± 11.25) ng·mg-1, P < 0.0005; (196.66 ± 14.9) ng·mg-1vs (74.43 ± 5.33) ng·mg-1, P < 0.0001, respectively] were reduced in liver tissues of diabetic rats. The IGF-1 gene downregulation varied with glucose control level of the diabetic state, and deteriorated gradually further with duration of diabetes. By month 6, hepatic tissue IGF-1mRNA was 0.71 ± 0.024 vs 1.12 ± 0.056, P < 0.001; 0.47 ± 0.021 vs 1.12 ± 0.056, P < 0.0005, respectively. IGF-1 peptide was (114.35 ± 8.09) ng·mg-1vs (202.05 ± 15.73) ng·mg-1, P < 0.0005; (64.58 ± 3.89) ng·mg-1vs (202.05 ± 15.73) ng·mg-1, P < 0.0001 respectively. Serum IGF-1 was also lowered in diabetic group with poor control of blood glucose. On week 2, serum IGF-1 concentrations were (371.0 ± 12.5) ng·mg-1vs (511.2 ± 24.7) ng·mg-1, P < 0.0005, (223.2 ± 9.39) ng·mg-1vs (511.2 ± 24.7) ng·mg-1, P < 0.0001 respectively. By month 6, (349.6 ± 18.62) ng·mg-1vs (520.7 ± 26.32) ng·mg-1, P < 0.0005, (188.5 ± 17.35 vs 520.7 ± 26.32) ng·mg-1, P < 0.0001, respectively. Serum IGF-1 peptide change was significantly correlated with that in liver tissue (r = 0.99, P < 0.001). Furthermore, No difference was found in the above parameters between diabetic rats with euglycemia and non-diabetic control group.

CONCLUSION: The influence of diabetic status on IGF-1 gene expression in liver tissues is started from early diabetic stage, causing down regulation of IGF-1 expression, and progresses with the severity and duration of diabetic state. Accordingly serum IGF-1 level decreases. This might indicate that liver tissue IGF-1 gene expression is greatly affected in diabetes, thus contributing to reduction of serum IGF-1 level.

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