Copyright
©The Author(s) 2004.
World J Gastroenterol. Jul 1, 2004; 10(13): 1923-1927
Published online Jul 1, 2004. doi: 10.3748/wjg.v10.i13.1923
Published online Jul 1, 2004. doi: 10.3748/wjg.v10.i13.1923
Gene | Quantification method | Primer sequence | cDNA |
NOS II | Forward primer | 5’-CCCTTCCGAAGTTTCTGGCAGCAG-3’ | 2793-2817 |
Reverse primer | 5’-GGGCTCCTCCAAGGTGTTGCCC-3’ | 3424-3446 | |
Probe | 5’-(FAM)TCTTCGGTGCGGTCTTTTCCTATGGAGCAA(TAMRA)-3’ | 3391-3421 | |
NOS III | Forward primer | 5’-CAAGACCGATTACACGACAT-3’ | 31-51 |
Reverse primer | 5’-GCTGGTTGCCATAGTGACAT-3’ | 300-321 | |
Probe | 5’-(FAM)GAGTGTTTGGACAAGTCCTCACCGCCTTTT-(TAMRA)-3’ | 158-188 | |
GAPDH | Forward primer | 5’-GAAGGTGAAGGTCGGAGTCA-3’ | |
Reverse primer | 5’-GAAGATGGTGATGGGA-3’ | ||
Probe | 5’-(JOE)CAAGCTTCCCGTTCTCAGCC(TAMRA)-3’ |
Stage | Index | Tetrandrine | Cirrhosis | Negative control |
(n = 8) | (n = 8) | (n = 8) | ||
Earlier period | PVF | 18.37 ± 1.83 | 21.25 ± 1.76 | 16.50 ± 1.73 |
PVP | 1.35 ± 0.25b | 1.67 ± 0.21 | 1.18 ± 0.13 | |
ICVP | 0.31 ± 0.04 | 0.32 ± 0.04 | 0.31 ± 0.04 | |
PVR | 5.73 ± 0.88 | 6.38 ± 1.12 | 5.81 ± 1.04 | |
Metaphase | PVF | 14.50 ± 1.61a | 20.35 ± 1.07 | 17.75 ± 1.37 |
PVP | 1.44 ± 0.13b | 1.72 ± 0.69 | 1.30 ± 0.21 | |
ICVP | 0.32 ± 0.04 | 0.31 ± 0.04 | 0.32 ± 0.04 | |
PVR | 7.98 ± 1.68 | 9.56 ± 1.29 | 5.53 ± 0.67 | |
Late period | PVF | 15.85 ± 1.23 | 17.57 ± 1.93 | 15.42 ± 1.27 |
PVP | 1.45 ± 0.24b | 1.67 ± 0.23 | 1.37 ± 0.10 | |
ICVP | 0.31 ± 0.04 | 0.32 ± 0.04 | 0.32 ± 0.04 | |
PVR | 7.44 ± 1.08 | 8.01 ± 0.79 | 6.76 ± 0.34 |
Stage | Index | Tetrandrine | Cirrhosis | Negative control |
(n = 8) | (n = 8) | (n = 8) | ||
Earlier period | NOS II | 0.1723 ± 0.0014 | 0.5635 ± 0.0104 | - |
NOS III | 0.2578 ± 0.0076 | 0.2694 ± 0.0043 | 0.2786 ± 0.0107 | |
Metaphase | NOS II | 0.1679 ± 0.0093 | 0.7521 ± 0.0098 | - |
NOS III | 0.3210 ± 0.0084 | 0.3412 ± 0.0113 | 0.2916 ± 0.0089 | |
Late period | NOS II | 0.1876 ± 0.0065 | 0.6987 ± 0.0078 | - |
NOS III | 0.2917 ± 0.0102 | 0.3002 ± 0.0091 | 0.2815 ± 0.0098 |
- Citation: Wang H, Chen XP, Qiu FZ. Increased hepatic expression of nitric oxide synthase type II in cirrhotic rats. World J Gastroenterol 2004; 10(13): 1923-1927
- URL: https://www.wjgnet.com/1007-9327/full/v10/i13/1923.htm
- DOI: https://dx.doi.org/10.3748/wjg.v10.i13.1923