Role of inducible nitric oxide synthase expression in aberrant crypt foci-adenoma-carcinoma sequence

Figure 1 Immunohistochemical analysis for iNOS protein in ACF. (A) weak staining was observed in the cytoplasm of nonhyperplastic ACF. (SP method, × 200). (B) weak staining was observed in the cytoplasm of hyperplastic ACF. (SP method, × 200). (C) strong staining was observed in the cytoplasm of dysplastic ACF. (SP method, × 200).

Figure 2 Histochemical detection of apoptosis by TUNEL in ACF. Few apoptotic cells were detected in nonhyperplastic ACF (Panel A, original magnification × 200), and more apoptotic cells were detected in hyperplastic ACF (Panel B, original magnification × 100) whereas they decreased significantly after transition to dysplastic ACF (Panel C, original magnification × 200).

Figure 3 Immunohistochemistry of PCNA protein in ACF. PCNA expression was gradually increased from nonhyperplastic ACF (Panel A, original magnification × 200), hyperplastic ACF (Panel B, original magnification × 100) to dysplastic ACF (Panel C, original magnification × 100).

Figure 4 Immunohistochemistry of CD34 protein in ACF. MVD determined by anti-CD34 antibody was low in nonhyperplastic ACF (Panel A, original magnification × 200) and hyperplastic ACF (Panel B, original magnification × 200) whereas significantly increased in dysplastic ACF (Panel C, original magnification × 200).