A small yeast RNA inhibits HCV IRES mediated translation and inhibits replication of poliovirus in vivo

Figure 1 Detection IRNA or mIRNA in the total RNA of HHCCs which constitutionally expressing IRNA or mIRNA. 1: DNA Marker DL2000; 2 and 3: RT-PCR of cell lines expressing IRNA; 4: RT-PCR of cell lines expressing mIRNA; 5 and 6: PCR of cell lines expressing IRNA or mIRNA.

Figure 2 The translation level of β-actin in HHCC and IRNA-expressing lines. (Laser confocal, 400 × 1.00). A: IRNA-express-ing cell lines, B: Control cell lines.

Figure 3 The effect of long-term expressing IRNA on cap-independent and cap-dependent protein translation.

Figure 4 The cytopathic effect of different HHCC cells infected with PV. A: control HHCC cells; B: mIRNA cells; C: pcDNA3 cells; D: IRNA cells.

Figure 5 Plaque characteristic of different HHCC infected of PV. A: control HHCC; B: mIRNA cells; C: pcDNA3 cells; D: IRNA cells.

Figure 6 IRNA forms a gel-retarded complex which is inhibited by 5’ UTR of HCV RNA. ³²P-labeled IRNA was incubated with RRL extract in the absence (lane 2) or presence of an unla-beled 25- or 50-fold molar excess of IRNA (lane3 and lane 4, respectively) or 5’ UTR (lane 5) or of a 50-fold molar excess of nonspecific RNA (lane 6). Protein-RNA complexes were analyzed on a nondenaturing gel. In lane 1, RRL extract was not added. FP: free probe; C: complexes.