A small yeast RNA inhibits HCV IRES mediated translation and inhibits replication of poliovirus in vivo

doi:10.3748/wjg.v9.i5.1008

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May 15, 2003 (publication date) through Nov 3, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. May 15, 2003; 9(5): 1008-1013
Published online May 15, 2003. doi: 10.3748/wjg.v9.i5.1008

A small yeast RNA inhibits HCV IRES mediated translation and inhibits replication of poliovirus in vivo

Xue-Song Liang, Jian-Qi Lian, Yong-Xing Zhou, Qing-He Nie, Chun-Qiu Hao

Xue-Song Liang, Jian-Qi Lian, Yong-Xing Zhou, Qing-He Nie, Chun-Qiu Hao, The center of diagnosis and treatment for infectious diseases of Tangdu Hospital of Military Medical University of PLA, Xi’an 710038, Shaanxi Province, China

Author contributions: All authors contributed equally to the work.

Supported by the Youth Natural Scientific Foundation of China, No. 30000147

Correspondence to: Dr Jian-Qi Lian, The center of diagnosis and treatment for infectious diseases of Tangdu Hospital of Military Medical University of PLA, Xi’an 710038, Shaanxi Province, China. lianjq@sohu.com

Telephone: +86-29-3377595

Received: November 19, 2002
Revised: November 27, 2002
Accepted: December 20, 2002
Published online: May 15, 2003

Abstract

AIM: To investigate the anti-virus infection activity of internal ribosome entry site (IRES) specific inhibitor RNA (IRNA).

METHODS: IRNA eukaryotic vector pcRz-IRNA or mIRNA eukaryotic vector pcRz-mIRNA was tansfected into human hepatocarcinoma cells (HHCC), then selected with neomycin G418 for 4 to 8 weeks, and then infected with polio virus vaccinas line. The cytopethogenesis effect was investigated and the cell extract was collected. At last the polio virus titer of different cells was determined by plaque assay.

RESULTS: Constitutive expression of IRNA was not detrimental to cell growth. HCV IRES-mediated cap-independent translation was markedly inhibited in cells constitutively expressing IRNA compared to control hepatoma cells. However, cap-dependent translation was not significantly affected in these cell line. Additionally, HHCC cells constitutively expressing IRNA became refractory to infection of polio virus.

CONCLUSION: IRES specific IRNA can inhibit HCV IRES mediated translation and poliovirus replication.

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