Copyright
©The Author(s) 2002.
World J Gastroenterol. Apr 15, 2002; 8(2): 353-356
Published online Apr 15, 2002. doi: 10.3748/wjg.v8.i2.353
Published online Apr 15, 2002. doi: 10.3748/wjg.v8.i2.353
Figure 1 Amino acid sequence alignment of HPO-205 and HPO (GenBank CI 11559825)
Figure 2 The mRNA expression of HPO-205 was determined by RT-PCR in hepatoma cell lines.
a: HepG2 cell line; b: HLE cell line; c: SMMC-7721 cell line; d: Bel-7402 cell line; GAPDH as a control.
Figure 3 HPO-205 protein was detected by Western blot in hepatoma cell lines.
a: HepG2 cell line; b: Bel-7402 cell line; c: SMMC-7721 cell line; d: HLE cell line
Figure 4 A: The HPO-205 mRNA expression in transfected Cos7 cells was detected by RT-PCR method.
a: DNA marker; b: pcDNAHPO-205 vector; c: pcDNA vector; B: The effect of DNA synthesis of HepG2 cells stimulated by the different expressed protein.
Figure 5 The stably transfected cell lines were confirmed and MAPK activation was examined by Western Blot.
A. HPO and HPO-205 protein in transfected Bel-7402 cells; B. MAPK levels in different transfected cell lines.
- Citation: Lu J, Xu WX, Zhan YQ, Cui XL, Cai WM, He FC, Yang XM. Identification and characterization of a novel isoform of hepatopoietin. World J Gastroenterol 2002; 8(2): 353-356
- URL: https://www.wjgnet.com/1007-9327/full/v8/i2/353.htm
- DOI: https://dx.doi.org/10.3748/wjg.v8.i2.353