Experimental and clinic-opathologic study on the relationship between transcription factor Egr-1 and esophageal carcinoma

Figure 1 Identification of PCMV-Egr-1 plasmid. 1. Marker; 2. Uncut plasmid; 3. Cut with Sac II, showing 7.6 kp fragment of whole plasmid; 4. Cut with Sac II and Sma I, showing 5.5 kp fragment of vector and 2.1 kp fragment of Egr-1 gene.

Figure 2 PCR amplification of neogene. 1. Marker; 2-5. For transfected Eca109, showing 327 bp of neo-gene; 6. Negative control Eca109, no band was shown.

Figure 3 Positive Egr-1 protein in nuclei of transfected Eca109 cells. ICC × 400

Figure 4 Cell growth curves showing lower growth rate in transfected Eca109 cell than in the control Eca109 cell.

Figure 5 The tumors of transfected Eca109 are smaller than that of control Eca109, in vitro, 30 d after injection in tumorigenicity test in SCID mice.

Figure 6 Egr-1 protein expression in basal mucosal layer in simple hyperplastic epithelia of esophagus. IHC × 200

Figure 7 Positive Egr-1 mRNA in cytoplasm of esophageal squamous cell carcinoma. ISH × 400