Transepithelial transport of putrescine across monolayers of the human intestinal epithelial cell line, Caco-2

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Figure 1 Apical vs. basolateral uptake of putrescine in confluent (day 7) and differentiated (day 14) Caco-2 cells in culture. The experiments were carried out as indicated in the Methods section. EGF (100 μg/L) was added to the basolateral chamber, and the cells were incubated for 12 h. Mean ± SEM, N = 4. ^aP < 0.001 vs control.

Figure 2 Transepithelial permeability of putrescine across Caco-2 cell monolayers. The experiments were done as described in Methods section, with 100 μM (0.1 μCi/mL) putrescine added to the apical chamber and incubation time 60 min. Mean ± SEM, N = 4.

Figure 3 Apparent permeability coefficients for mannitol and putrescine. Each bar represents mean ± SEM of six experiments. Differences between the values are not statistically significant.

Figure 4 Putrescine release from Caco-2 cells pre-loaded with physiological concentrations (100 μmol/sample) putrescine ± EGF. Each data point represents mean ± SEM of six experiments.