Expression characteristics of peripheral lymphocyte programmed death 1 and FoxP3+ Tregs in gastric cancer during surgery and chemotherapy

Figure 1 Programmed death 1 expression on all T cell subsets of healthy donors and preoperative patients with gastric cancer. A: The scatter and histogram plots of one representative patient are illustrated. The relationships between the diagrams are indicated by arrows and gates (R1, R2, and R3); B-G: The percentages of total programmed death 1⁺ (PD-1⁺) cells (B), CD4⁺PD-1⁺ (C), CD8⁺PD-1⁺ (D), CD45RO⁺PD-1⁺ (E), CD4⁺CD45RO⁺PD-1⁺ (F), and CD8⁺CD45RO⁺PD-1⁺ (G) cells out of the total peripheral lymphocytes from patients and healthy donors are shown. The P value was calculated using a Student’s t-test or Wilcoxon rank sum test.

Figure 2 Changes in programmed death 1 expression on all subsets before and after the D2 gastrectomy. A-F: The percentage of total programmed death 1⁺ (PD-1⁺) cells (A), CD4⁺PD-1⁺ (B), CD8⁺PD-1⁺ (C), CD45RO⁺PD-1⁺ (D), CD4⁺CD45RO⁺PD-1⁺ (E), and CD8⁺CD45RO⁺PD-1⁺ (F) cells of the peripheral lymphocytes derived from patients before and after the D2 gastrectomy are shown. The P value was calculated using an analysis of variance repeated-measures function.

Figure 3 Changes in programmed death 1 expression on all subsets during chemotherapy. A-F: The percentage of total programmed death 1⁺ (PD-1⁺) cells (A), CD4⁺PD-1⁺ (B), CD8⁺PD-1⁺ (C), CD45RO⁺PD-1⁺ (D), CD4⁺CD45RO⁺PD-1⁺ (E), and CD8⁺CD45RO⁺PD-1⁺ (F) cells of the peripheral lymphocytes derived from patients during chemotherapy are shown. The P value was calculated using an analysis of variance repeated-measures function.

Figure 4 Treg and activated Treg cells in the peripheral blood of donors and patients. A: Scatter plots are shown from one representative patient. Relationships of the diagrams are denoted with arrows and gates; B-G: The percent of TCD4⁺CD25⁺FoxP3⁺ and CD4⁺CD45RO⁺FoxP3^high cells in the peripheral lymphocytes from the donors and patients during treatment were shown and the P value was calculated using an analysis of variance repeated-measures function.

Figure 5 Relative programmed death 1 expression on lymphocytes and the percent of Treg and activated Treg cells. A and B: Programmed death 1 expression on lymphocytes and the percent of CD4⁺CD25⁺FoxP3⁺ (A) and CD4⁺CD45RO⁺FoxP3^high (B) cells in the peripheral blood. PD-1: Programmed death 1.