Long noncoding RNA HOXA11-AS promotes gastric cancer cell proliferation and invasion via SRSF1 and functions as a biomarker in gastric cancer

Figure 1 HOXA11-AS expression in GC tissues, cell lines, and serum samples. A: Relative expression of HOXA11-AS in gastric cancer and normal tissues; B: Relative levels of serum HOXA11-A in gastric cancer patients and healthy controls; C: Relative levels of HOXA11-A in MNK-45 and GES-1 cells. The assays were repeated three times. ^bP < 0.01, ^cP < 0.001. GC: Gastric cancer.

Figure 2 Performance of HOXA11-AS in the diagnosis of gastric cancer. A: Serum HOXA11-AS levels in the samples obtained 1 d and 1 mo after surgery; B: The ROC curve demonstrates that serum HOXA11-AS can discriminate gastric cancer patients from healthy controls (AUC = 0.924, 95%CI: 0.881-0.967, P = 0.001) with a sensitivity of 78.7% and specificity of 97.8%. ^cP < 0.001. The assays were repeated three times. PreOp: Pre-operation; 1 d: The first day after surgical removal of the tumor; 1 mo: The 30^th day after surgical removal of the tumor; ROC: Receiver operating characteristic curve; AUC: Area under the curve.

Figure 3 Kaplan-Meier survival curves demonstrating that gastric cancer patients with low HOXA11-AS expression has a better overall survival rate. The assays were repeated three times.

Figure 4 Transfection efficiencies and cell function assays. A and B: Transfection efficiencies of pcDNA-HOXA11-AS (A) and HOXA11-AS-siRNA (B) in gastric cancer cells; C and D: Migration of MNK-45 cells after HOXA11-AS overexpression (C) or knockdown (D); E and F: Invasion of MNK-45 cells after HOXA11-AS overexpression (E) or knockdown (F). The assays were repeated three times. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. OD: Optical density; NC: Normal control.

Figure 5 HOXA11-AS regulates expression of SFSR1 in gastric cancer cells. A: Protein expression of SFSR1 after overexpression of HOXA11-AS in gastric cancer (GC) cells. B: Protein expression of SFSR1 after knockdown of HOXA11-AS in GC cells. The assays were repeated three times. ^aP < 0.05, ^cP < 0.001. NC: Normal control.