Increased CD4+CD45RA-FoxP3low cells alter the balance between Treg and Th17 cells in colitis mice

Figure 1 Treg cell features in multiple samples including peripheral blood monocytes, spleen, mesenteric lymph node, lamina propria of jejunum and colon. A: Representative FACS analysis of Treg cells from HC and UC mice samples gated for CD4⁺ T cells; B: Treg cell percentages in HC and UC samples; C: Expression of FoxP3 mRNA extracted from Treg cells in HC and UC samples; D: Levels of IL-10 secreted from mononuclear cells from HC and UC peripheral blood monocytes (PBMC), spleen, mesenteric lymph node (MLN), LPJ, and LPC samples; E: Treg cell distribution in HC and UC samples. ^aP < 0.05 vs control, ^bP < 0.01 vs control, ^cP < 0.001 vs control, ^dP < 0.05 vs LPC, ^eP < 0.01 vs LPC, ^fP < 0.001 vs LPC. HC: Healthy control mice; UC: Ulcerative colitis mice; LPJ: Lamina propria of jejunum; LPC: Lamina propria of colon.

Figure 2 T helper 17 cell characteristics in healthy control mice and ulcerative colitis mice peripheral blood monocytes, spleen, mesenteric lymph node, lamina propria of jejunum and colon samples. A: FACS analysis of Th17 cells in HC and UC samples gated for CD4⁺ T cells; B: Percentages of Th17 cells in different HC and UC samples; C: Levels of IL-17A secreted by mononuclear cells extracted from HC and UC peripheral blood monocytes (PBMC), mesenteric lymph node (MLN), LPJ, and LPC samples; D: Treg cell distribution in HC and UC samples. ^aP < 0.05 vs control, ^bP < 0.01 vs control, ^cP < 0.001 vs control, ^dP < 0.05 vs LPC, ^eP < 0.01 vs LPC, ^fP < 0.001 vs LPC, ^gP < 0.001 vs LPJ. HC: Healthy control mice; UC: Ulcerative colitis mice; LPJ: Lamina propria of jejunum; LPC: Lamina propria of colon.

Figure 3 Treg cell subset characteristics in healthy control mice and ulcerative colitis mice peripheral blood monocytes, spleen, mesenteric lymph node, lamina propria of jejunum, and colon samples. A: FACS analysis of Treg cell subsets in HC and UC samples gated for CD4⁺ T cells; B: FoxP3 mRNA expression by FrIII cells from different HC and UC samples; C: Expression levels of IL-17A mRNA by FrIII cells in HC and UC samples; D: RORC mRNA expression by FrIII cells from HC and UC samples; E: Treg cell subset distribution in UC samples. ^aP < 0.05 vs control, ^bP < 0.01 vs control, ^cP < 0.001 vs control, ^dP < 0.05 vs LPC, ^eP < 0.01 vs LPC, ^fP < 0.001 vs LPC. PBMC: Peripheral blood monocytes; MLN: Mesenteric lymph node; FrI cells: CD4⁺CD45RA⁺FoxP3^low; FrII cells: CD4⁺CD45RA^-FoxP3^high; FrIII cells: CD4⁺CD45RA^-FoxP3^low; HC: Healthy control mice; UC: Ulcerative colitis mice; LPJ: Lamina propria of jejunum; LPC: Lamina propria of colon.

Figure 4 FoxP3⁺IL17A⁺ and FoxP3⁺IL17A^- cell characteristics in healthy control mice and ulcerative colitis mice samples of peripheral blood monocytes, spleen, mesenteric lymph node, lamina propria of jejunum, and colon. A: FACS analysis of FoxP3⁺IL17A⁺ and FoxP3⁺IL17A^- cells in HC and UC samples gated for CD4⁺CD25⁺ T cells; B: Percentage of FoxP3⁺IL17A⁺ cells in HC and UC samples; C: Percentages of FoxP3⁺IL17A^- cells in HC and UC samples; D: FoxP3⁺IL17A⁺ cell distribution in different HC and UC samples. ^aP < 0.05 vs control, ^bP < 0.01 vs control, ^cP < 0.001 vs control, ^dP < 0.05 vs LPC, ^eP < 0.01 vs LPC, ^fP < 0.001 vs LPC. PBMC: Peripheral blood monocytes; MLN: Mesenteric lymph node; FrI cells: CD4⁺CD45RA⁺FoxP3^low; FrII cells: CD4⁺CD45RA^-FoxP3^high; FrIII cells: CD4⁺CD45RA^-FoxP3^low; HC: Healthy control mice; UC: Ulcerative colitis mice; LPJ: Lamina propria of jejunum; LPC: Lamina propria of colon.