Overexpression of Csk-binding protein decreases growth, invasion, and migration of esophageal carcinoma cells by controlling Src activation

Figure 1 Overexpression of Csk-binding protein decreases the expression of Src in TE-1 cells. Western blot analysis of the expression of Csk-binding protein (CBP) and Src in TE-1 cells before and after transduction with CBP lentiviral vectors. GAPDH was used as a protein loading control. The expression of CBP was much higher in transduced TE-1 cells than in normal TE-1 and TE-1/pLenO-DCE cells (P < 0.05), and expression of Src was much lower in transduced TE-1 cells than in normal TE-1 and TE-1/pLenO-DCE cells (P < 0.05). 1 = normal TE-1 cells; 2 = TE-1 cells after transfection with pLenO-DCE-(-); 3 = TE-1 cells after overexpression CBP.

Figure 2 Cell growth of TE-1, TE-1/pLenO-DCE-(-) and TE-1/Csk-binding protein cells. The growth of TE-1/CBP cells was slower than that of TE-1 and TE-1/pLenO-DCE-(-) cells (P < 0.05).

Figure 3 Invasion of TE-1, TE-1/pLenO-DCE-(-) and TE-1/Csk-binding protein cells. Trypan blue staining showed that TE-1, TE-1/pLenO-DCE-(-) and TE-1/CBP cells passed through the Matrigel (200×). ^aP < 0.05 vs TE-1/CBP cells, no significant difference compared with TE-1/pLenO-DCE-(-) cells.

Figure 4 Migration of TE-1, TE-1/pLenO-DCE-(-) and TE-1/ Csk-binding protein cells. The scratch assay showed that TE-1, TE-1/pLenO-DCE-(-) and TE-1/CBP cells moved into the scratch (200×). The distance of cell migration was assessed. ^aP < 0.05 vs TE-1/CBP cells, no significant difference compared with TE-1/pLenO-DCE-(-) cells.