Effects of Institut Georges Lopez-1 and Celsior preservation solutions on liver graft injury

Figure 1 Concentration-response curve for acetylcholine in rat hepatic arterial rings pre-contracted with phenylephrine: maximal effect (Emax) and EC₅₀ values. Control (n = 12): hepatic artery rings were reoxygenated in the organ bath without cold storage. IGL-1 (n = 11): hepatic artery rings were reoxygenated in the organ bath after cold preservation in IGL-1 solution. Celsior (n = 11): hepatic artery rings were reoxygenated in the organ bath after cold preservation in Celsior solution. Values are means ± SD. ^aP < 0.05 vs Control. IGL-1: Institut Georges Lopez-1.

Figure 2 Concentration-response curves for acetylcholine in rat hepatic arterial rings precontracted with phenylephrine in presence of L-NAME (A) and after pretreatment with L-NAME + indomethacin (B). Control (n = 12): hepatic artery rings were reoxygenated in the organ bath without cold storage and pretreated with L-NAME. IGL-1 (n = 11): hepatic artery rings were reoxygenated in the organ bath after cold preservation in IGL-1 solution and pretreatment. Celsior (n = 11): hepatic artery rings were reoxygenated in the organ bath after cold preservation in Celsior solution and pretreatment. ^aP < 0.05 vs Control + L-NAME, ^cP < 0.05 vs IGL-1 + L-NAME. IGL-1: Institut Georges Lopez-1.

Figure 3 Alanine aminotransferase and aspartate aminotransferase activities in flushing effluent after 24 h of ischemic cold preservation (A, B) and after 2 h of normothermic reperfusion (C, D). Control (n = 6): livers were flushed and perfused ex vivo without cold storage. IGL-1 (n = 6): livers were preserved in IGL-1 solution at 4 °C for 24 h. Celsior (n = 6): livers were preserved in Celsior solution at 4 °C for 24 h. ^aP < 0.05 vs Control, ^cP < 0.05 vs IGL-1. IGL-1: Institut Georges Lopez-1.

Figure 4 Liver function expressed as bile flow (A) and bromosulfophthalein clearance (B) and oxidative stress expressed as hepatic malondialdehyde concentration (C) and catalase activity (D) after 2 h of normothermic reperfusion. Control (n = 6): livers were flushed and perfused ex vivo without cold storage. IGL-1 (n = 6): livers were preserved in IGL-1 solution at 4 °C for 24 h. Celsior (n = 6): livers were preserved in Celsior solution at 4 °C for 24 h. ^aP < 0.05 vs Control, ^cP < 0.05 vs IGL-1. IGL-1: Institut Georges Lopez-1.

Figure 5 Phosphorylated AMP activated protein kinase (A) and AKT levels (B) and endothelial nitric oxide synthase protein (C) levels and nitrate and nitrite contents (D) in liver tissues after 2 h of warm reperfusion. The upper panels show one representative blot of three independent experiments and the lower panels show densitometric evaluation of the protein blot. Control (n = 6): livers were flushed and perfused ex vivo without cold storage. IGL-1 (n = 6): livers were preserved in IGL-1 solution at 4 °C for 24 h. Celsior (n = 6): livers were preserved in Celsior solution at 4 °C for 24 h. ^aP < 0.05 vs Control, ^cP < 0.05 vs IGL-1. IGL-1: Institut Georges Lopez-1.

Figure 6 Phosphorylated c-Jun N-terminal kinase protein (A), ERK protein (B) and p38 protein (C) levels after two hours of normothermic reperfusion. The upper panels show one representative blot of three independent experiments and the lower panels show densitometric evaluation of the protein blot. Control (n = 6): livers were flushed and perfused ex vivo without cold storage. IGL-1 (n = 6): livers were preserved in IGL-1 solution at 4 °C for 24 h. Celsior (n = 6): livers were preserved in Celsior solution at 4 °C for 24 h. ^aP < 0.05 vs Control, ^cP < 0.05 vs IGL-1. IGL-1: Institut Georges Lopez-1.