IGF2 differentially methylated region hypomethylation in relation to pathological and molecular features of serrated lesions

Figure 1 Distribution of IGF2 differentially methylated region 0 methylation levels in 351 serrated lesions. Hyperplastic polyp, sessile serrated adenoma (SSA), SSA with cytological dysplasia, traditional serrated adenoma (TSA) and TSA with high-grade dysplasia (HGD) and 185 non-serrated adenomas (tubular adenoma, tubular adenoma with HGD, tubulovillous adenoma and tubulovillous adenoma with HGD). DMR: Differentially methylated region; IGF2: Insulin-like growth factor 2.

Figure 2 IGF2 differentially methylated region 0 methylation level according to histological type. Insulin-like growth factor 2 (IGF2) differentially methylated region (DMR)0 methylation levels of sessile serrated adenoma (mean ± SD; 73.1 ± 12.3) were significantly higher compared with those of hyperplastic polyp (61.9 ± 20.5, P < 0.0001), traditional serrated adenoma (61.6 ± 19.6, P < 0.0001), and non-serrated adenoma (59.0 ± 15.8, P < 0.0001). P-values were calculated by analysis of variance.

Figure 3 Kaplan-Meier survival curves for colorectal cancer according to the IGF2 differentially methylated region 0 and long interspersed nucleotide element-1 methylation levels in metastatic colorectal cancers. A: Patients with Insulin-like growth factor 2 (IGF2) differentially methylated region (DMR)0 hypomethylation had a slightly higher mortality rate than those with IGF2 DMR0 hypermethylation, but this difference was not significant (log-rank test: P = 0.13); B: IGF2 DMR0 hypomethylation (Q4 cases) was significantly associated with unfavorable cancer-specific survival (log-rank test: P = 0.038); C: Significantly higher mortality was observed in patients with long interspersed nucleotide element-1 (LINE-1) hypomethylation compared with those with LINE-1 hypermethylation (log-rank test: P = 0.026); D: LINE-1 hypomethylation (Q4 cases) was significantly associated with unfavorable cancer-specific survival (log-rank test: P = 0.0037).