Plasma free amino acid profiling of esophageal cancer using high-performance liquid chromatography spectroscopy

Figure 1 Chromatogram of 19 amino acids. Retention times of 6.1, 7.7, 10.1, 12.4, 12.8, 13.8, 14.1, 17.0, 17.3, 17.8, 18.2, 23.9, 24.1, 25.0, 25.8, 27.9, 28.3, 28.5 and 29.4 min correspond to aspartate (Asp), glutamate (Glu), asparagine (Asn), serine (Ser), glycine (Gly), histidine (His), arginine (Arg), threonine (Thr), taurine (Tau), alanine (Ala), proline (Pro), cysteine (Cys), tyrosine (Tyr), valine (Val), methionine (Met), lysine (Lys), isoleucine (Ile), leucine (Leu), and phenylalanine (Phe), respectively.

Figure 2 Profiling of high-performance liquid chromatography spectra from patients with ESCC and healthy donors by partial least squares-discriminant analysis. Partial least squares-discriminant analysis (PLS-DA) model with plasma of ESCC subjects (filled square) and healthy subjects (filled triangle). Model parameters r²x = 0.53, r²y = 0.58, and Q² = 0.53.