Response to Abadi and Kusters, World J Gastroenterol 19: 429-430

Figure 1 Location of inducible nitric oxide synthetase-specific primer annealing sites. The sequence of exon 16 of the inducible nitric oxide synthetase (iNOS) gene (http://www.ncbi.nlm.nih.gov/nuccore/x85772) is shown. The sequence of forward primer used in our study and by Shen et al[3] is in bold italics at the 5’ end of the sequence above: 5’-TGTAAACCAACTTCCGTGGTG-3’. The sequence of the reverse primer used in our study and by Shen et al[3] (5’-GTCTCTGCGGGTCTGAGAAG-3’) is the reverse compliment of the bold italicized bases in the 3’ end of the sequence above. The C150T polymorphism is indicated by the bold underlined “C”.