Effect of the ginsenoside Rb1 on the spontaneous contraction of intestinal smooth muscle in mice

Figure 1 Effect of Rb1 on spontaneous contraction of intestinal smooth muscle. A: The representative effects of Rb1 on spontaneous contraction of intestinal smooth muscle in a dose-dependent manner; B, C: The amplitude of spontaneous contraction was decreased after Rb1 administration, but the frequency was unaffected. Values are expressed as means ± SE. n = 5, ^aP < 0.05 vs control group.

Figure 2 Effect of Rb1 on spontaneous contraction of the intestinal smooth muscle. A: Effect of Rb1 on spontaneous contraction of the intestinal smooth muscle; B-F: Effects of Rb1 (50 μmol/L) on spontaneous contraction of the intestinal smooth muscle pretreated with TEA (10 mmol/L), 4-AP (0.5 mmol/L), TTX (0.4 μmol/L) and Glibenclamide (10 μmol/L) respectively. TEA: Tetraethylammonium; 4-AP: 4-aminopyridine; TTX: Tetrodotoxin.

Figure 3 Summary in effect of Rb1on spontaneous contraction. Summary in effect of Rb1on spontaneous contraction of normal intestinal smooth muscles and those pretreated with TEA (10 mmol/L), 4-AP (0.5 mmol/L), Glibenclamide (10 μmol/L) and TTX (0.4 μmol/L) respectively. Values are expressed as means ± SE. n = 5, ^aP < 0.05 vs control group; ^cP < 0.05 TEA + Rb1 or 4-AP + Rb1 vs Rb1 group. TEA: Tetraethylammonium; 4-AP: 4-aminopyridine; TTX: Tetrodotoxin.

Figure 4 Effect of Rb1 on the voltage-gated K⁺ channel current of intestinal smooth muscle cell in mouse. A: The raw trace; B: The I-V relation curve; C: Summary in effect of Rb1 (50 μmol/L) on the voltage-gated K⁺ channel current at +60 mV. Values are expressed as means ± SE. n = 6, ^aP < 0.05 vs control group.

Figure 5 Effect of Rb1 on spontaneous transient outward currents and ATP sensitive potassium channel current of intestinal smooth muscle cell in mouse (n = 5). A: The raw trace of Rb1-induced effect on STOC of intestinal smooth muscle cell; B: The raw trace of Rb1-induced effect on IK_ATP of intestinal smooth muscle cell; C: Relative current evoked by Rb1 (50 μmol/L) on STOC, n = 5, ^aP < 0.05 vs control group; D: Relative current evoked by Rb1 (50 μmol/L) on IK_ATP comparing with control group. Values are expressed as means ± SE, n = 3. IK_ATP: ATP sensitive potassium channel current; STOC: Spontaneous transient outward currents.