Antitumor bioactivity of adenovirus-mediated p27mt in colorectal cancer cell line SW480

Figure 1 p27mt sequencing assay of transductants. Sequencing result was consistent with p27mt sequence in gene bank. Sequencing result was shown and the underline was mutant site Met-187/Ile-188 (ATGATC).

Figure 2 Expression of p27, Bcl-2 and Bax protein in each transductant by Western blot analysis.

Figure 3 Growth of transductants in vitro. Relative cell number was evaluated by comparing the absorbance in each cell at day 2, 4 and 6. The values shown are the mean of eight determinations. ^aP < 0.05, vs control cells.

Figure 4 Cell cycle analysis of transductants by flow cytometry. A: SW480; B: SW480-ad-β-gal; C: SW480-ad-p27mt. C-F: Sub-G₁, G₀/G₁, S and G₂/M phase, respectively. The percentage of cells in sub-G₁, G₀/G₁, S and G₂/M phase is shown in Table 1.

Figure 5 Cell migration assay by Boyden chamber. After 24 h of cell incubation, the migrated cells in each transductant were counted in six different fields under a light microscope. ^aP = 0.004, vs control cells.

Figure 6 Upper: A, B and C indicated respectively the groups which received respectively intratumoral injections of ad-p27mt, ad-β-gal or PBS. Lower: In vivo tumor growth inhibition assay. The weight of tumors was measured. The values are mean of the weight of tumors of each group ^aP = 0.002, vs control groups.