Relationship between nm23H1 genetic instability and clinical pathological characteristics in Chinese digestive system cancer patients

Figure 1 PAGE of D17S396 locus and immunohistochemistry of nm23H1 protein in gastric cancer. A: No difference in tumor tissue (2C) and normal tissue (2N). MSI (arrow) was positive when an allele band was added (1C) as compared with normal tissue (1N); B: No difference in tumor tissue (2C) and normal tissue (2N). LOH was positive when an allele band was added (arrow) in tumor tissue (3C) as compared with normal tissue (3N); C: Brown-yellow granules of nm23H1 protein mostly located in cytoplasm. The stained nucleolus and membrane of cells were observed (arrow, × 200); D: Control group, PBS replaced anti-nm23H1 protein as the first antibody (× 400).

Figure 2 PAGE of D17S396 locus and immunohistochemistry of nm23H1 protein in colon cancer. A: No difference in tumor tissue (2C) and normal tissue (2N). MSI was positive when an allele band was added (arrow) in tumor tissue (1C) as compared with normal tissue (1N); B: No difference in tumor tissue (4C) and normal tissue (4N). LOH was positive when an allele band was added (arrow) in tumor tissue (3C) as compared with normal tissue (3N); C: Brown-yellow granules of nm23H1 protein mostly located in membrane. The stained nucleolus and cytoplasm of cells were observed (arrow, × 400); D: Control group, PBS replaced anti-nm23H1 protein as the first antibody (× 200).

Figure 3 PAGE of D17S396 locus and immunohistochemistry of nm23H1 protein in HCC. A: No difference in tumor tissue (2C) and normal tissue (2N). MSI was positive when an allele band was added (arrow) in tumor tissue (1C) as compared with normal tissue (1N); B: No difference in tumor tissue (4C) and normal tissue (4N). LOH was positive in the absence of an allele band (arrow) in tumor tissue (3C) as compared with normal tissue (3N); C: Brown-yellow granules of nm23H1 protein mostly located in cytoplasm. The stained nucleolus and membrane of cells were observed (arrow, × 400); D: Control group, PBS replaced anti-nm23H1 protein as the first antibody (× 200).

Figure 4 PAGE of D17S396 locus and immunohistochemistry of nm23H1 protein in gallbladder carcinoma. A: No difference in tumor tissue (2C) and normal tissue (2N). MSI was positive when an allele band was added (arrow) in tumor tissue (1C) as compared with normal tissue (1N); B: No difference in tumor tissue (4C) and normal tissue (4N). LOH was positive in the absence of an allele band (arrow) in tumor tissue (3C) as compared with normal tissue (3N); C: Brown-yellow granules of nm23H1 protein mostly located in cytoplasm, and part of the stained nucleolus and membrane of cells were observed (arrow, × 200); D: Control group, PBS replaced anti-nm23H1 protein as the first antibody (× 200).