Gastric cancer cell lines induced by trichostatin A

Figure 1 TSA (37. 5 ng/mL per 72 h in BGC-823 cells and 75 ng/mL per 72 h in SGC-7901 cells) induced apoptosis (Hoechst 33342 staining, × 400). A: No typical apoptotic BGC-823 cells were observed in control group; B: BGC-823 cells treated with TSA (37.5 ng/mL per 72 h) showed the typical apoptotic nuclear morphology; C: No typical apoptotic SGC-7901 cells were observed in control group; D: SGC-7901 cells treated with TSA (75 ng/mL for 72 h) showed the typical apoptotic nuclear morphology.

Figure 2 Effect of TSA on the cell cycle and apoptosis as analyzed by FACS. A: Control of BGC-823 cells; B: Treatment with 37.5 ng/mL TSA for 72 h in BGC-823 cells; C: Control of SGC-7901 cells; D: Treatment with 75 ng/mL TSA for 72 h in SGC-7901 cells.

Figure 3 Cell lysates were analyzed by Western blots using anti-GAPDH and anti-acetyl-histone H3 antibody. A: BGC-823 cells acetylated histone H3 (17 kDa); B: SGC-7901 cell acetylated histone H3 (17 kDa); C: BGC-823 cells GAPDH (loading control, 37 kDa); D: SGC-7901 cells GAPDH (loading control, 37 kDa).