Hepatitis B virus infection and replication in primarily cultured human fetal hepatocytes

Figure 1 Morphology, PAS and S-P staining of the HFHs under light microscope. A: Isolated HFHs formed epithelial-like spheroid and they were surrounded by fibroblast-like cells in culture after 48 h; B: HFHs were stained by PAS, the majorities of cultured cells were positive; C: HFHs state in vitro for 50 d; D: HFHs were positive for CK8 in hepatocytes in vitro for 50 d (S-P); E: HFHs expressed liver marker CK18 in vitro for 14 d (S-P); F: Only a few of hepatocytes were positive for CK18 in vitro for 50 d (S-P); G: HFHs expressed ALB in vitro for 14 d (S-P). (Original magnification, × 40).

Figure 2 Lactate dehydrogenase (LDH) released in primary human hepatocytes.

Figure 3 HBsAg (A) and HBeAg (B) in supernatant detected by ELISA (OD:optical denisity).

Figure 4 HBV-DNA in media detected by FQ-PCR.

Figure 5 Detection of HBcAg by immunohistochemistry staining (S-P). A: The HBcAg positive in hepatocytes; B: Negative control for HBcAg. Original magnification, × 40.