RhoA and RhoC -siRNA inhibit the proliferation and invasiveness activity of human gastric carcinoma by Rho/PI3K/Akt pathway

Figure 1 RhoA and RhoC siRNAs inhibit RhoA and RhoC protein levels with Western blots. Lanes 1: SGC7901 cells were untreated; lanes 2-4: or transfected with anti-RhoA or anti-RhoC, Assays were performed 24 h after the end of treatment. Cells were treated with RhoA siRNA (A), RhoC siRNA (B), or control siRNA (C), β-actin served as an internal control.

Figure 2 Anti-RhoA and anti-RhoC siRNAs inhibit RhoA and RhoC gene levels with RT-PCR. RhoA and RhoC mRNA levels were significantly reduced in the RhoA and RhoC siRNA line. The level of β-actin mRNA after 20 cycles was included as a control.

Figure 3 The values of 490 nm wavelength light absorption in the five group.