Inhibition of human gastric carcinoma cell growth by atofluding derivative N3-o-toluyl-fluorouracil

Figure 1 Metabolism of atofluding to into TFU and 5-FU. The acetyl group on the N1 position of atofluding was hydrolyzed in the presence of H₂O into TFU rapidly due to the influence of fluorine on the C5 position. 5-FU was transformed from TFU slowly and continually by the liver microsomal enzymes.

Figure 2 TFU inhibits MKN-45 (A) and SGC-7901 (B) cell growth in vitro. Cells were treated with TFU (0.1, 1, 10, 100, 1000 μg/mL) for up to 120 h. Viable cell numbers were evaluated by the MTT assay and expressed as percentage of untreated controls at the concurrent time point. The bars indicate means ± SD (n = 3).