Augmenter of liver regeneration promotes hepatocyte proliferation induced by Kupffer cells

Figure 1 Effects of ALR on proliferation of Kupffer cells and hepatocytes. A: DNA replication; B: BrdU-positive nuclei. KC: Kupffer cells; HC: Hepatocytes; KC to HC: Hepatocytes conditioned by Kupffer cells. ^aP < 0.05 vs control, n = 3.

Figure 2 BrdU immunohistochemistry. The nuclei of BrdU-positive cells were stained brown. A: Kupffer cells; B: Hepatocytes; C: Hepatocytes conditioned by Kupffer cells (× 100).

Figure 3 ³H-leucine incorporation assay. A: Kupffer cells and associated medium; B: Hepatocytes and associated medium; C: Hepatocytes conditioned by Kupffer cells and associated medium. ^aP < 0.05 vs control, n = 3.

Figure 4 ¹²⁵I-rrALR binding assay. A: Saturation cures of ¹²⁵I-rrALR binding to its receptor on Kupffer cells; B: Scatchard plot of the ALR receptor on Kupffer cells; C: Binding of ALR to cultured hepatocytes (mean ± SD, n = 3).