Hot water-extracted Lycium barbarum and Rehmannia glutinosa inhibit proliferation and induce apoptosis of hepatocellular carcinoma cells

Figure 1 Representative DNA histograms (A) and percentage of cells in different cell cycle phases (B) after incubated with various concentrations of hot water-extracted LBE and RGE for 24 h in rat H-4-II-E cells determined by flow cytometry. Values are mean ± SD (n = 6).

Figure 2 Expression of p53 protein with the molecular weight of 53 ku visualized by Western blotting (A) and quantitated by an image analysis system (B) after incubation of rat H-4-II-E cells with various concentrations of hot water-extracted LBE and RGE for 24 h. Samples were pooled from 3 independent experiments (n = 3). Alpha-tubulin (55 ku) was used as an internal control.