Generation and characterization of a transgenic mouse model for pancreatic cancer

Figure 1 PCR results of transgenic mice. M: DL 2000 marker; C: Non-transgenic mouse concrol; P: pBC-SV40Tag plasmid; 34 and 37: the number of gene positive mice.

Figure 2 Southern blotting of transgenic mice. +: pBC-SV40Tag plasmid positive control; 34 and 37: the number of gene positive mice.

Figure 3 Appearance and anatomy of transgenic mice. A: Transgenic mice showing ridgy belly (arrowhead); B: Transgenic mice showing a tumor in its abdominal cavity; C and D: Abdominal cavity and tumor excised from the body.

Figure 4 Normal pancreas and tumor tissues-stained with H&E. A, B and C: Tissue of cystic neoplams from transgenic mice (A: × 100; B: × 200; C: × 400); D: Normal pancreas with islet and acinar cell (× 200).

Figure 5 RT-PCR results of transgene expression in tumor. M: DL-2000 marker, 1: blank control (H₂O), 2: cDNA of tumor tissue, 3: negative control (non-reverse transcripted RNA).

Figure 6 SV40Tag expression in tumor tissue detected by immunohistochemistry without anti-Tag antibody (A) and with anti-Tag antibody (B).