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World J Gastroenterol. May 7, 2006; 12(17): 2785-2788
Published online May 7, 2006. doi: 10.3748/wjg.v12.i17.2785
Generation and characterization of a transgenic mouse model for pancreatic cancer
Qiang Sun, Jie Feng, Xiao-Luan Wei, Rong Zhang, Su-Zhen Dong, Qian Shen, Juan Dong, Hou-Da Li, Ying-He Hu
Jie Feng, Rong Zhang, Juan Dong, Hou-Da Li, Comparative Medical Center, Yangzhou University, Yangzhou 225009, Jiangsu Province , China
Qiang Sun, Xiao-Luan Wei, Su-Zhen Dong, Qian Shen, Ying-He Hu, Shanghai Institute of Brain Functional Genomics, East China Normal University, Shanghai, 200062, Shanghai, China
Co-correspondents: Hou-Da Li
Supported by the National Key Technologies Research and Development Program of China during The 10th Five-Year Plan Period , No 2001BA70113.
Correspondence to: Dr. Ying-He Hu, Shanghai Institute of Brain Functional Genomics, East China Normal University, Shanghai 200062, China. yhu@brain.ecnu.edu.cn
Telephone: +86-21-62232789 Fax: +86-21-62601953
Received: April 26, 2005
Revised: August 12, 2005
Accepted: December 12, 2005
Published online: May 7, 2006
Abstract

AIM: To generate a SV40Tag transgenic tumor animal model and to study the mechanism underlying tumorigenesis.

METHODS: A mammary gland expression vector containing SV40Tag DNA was generated. Transgene fragments were microinjeted into fertilized eggs of FVB mice. The genetically manipulated embryos were transferred into the oviducts of pseudo-pregnant female mice. PCR and Northern blot analysis were used for genotype analysis of F1 and F2 mice. Transgene expression was detected by RT-PCR and immunohistochemistry.

RESULTS: SV40Tag gene was detected in two lines of transgenic mice. One of them delivered the transgene to F1 and a tumor was found in the pancreas of these mice. RT-PCR and immunohistochemistry showed that SV40Tag gene was expressed in the tumor. Pathological characterization of the transgenic mice demonstrated that the tumor belonged to pancreatic cystic neoplasm.

CONCLUSION: SV40Tag transgenic mouse model can be successfully established. The transgenic mice develop a pancreatic tumor, which can be used for investigation of the molecular mechanism of tumorigenesis in vivo.

Keywords: SV40Tag; Transgenic mice; Animal model; Pancreatic neoplasms