Increase of CD4+CD25+ T cells in Smad3-/- mice

Figure 1 Total numbers and differential distributions of blood leukocytes in Smad3^+/+ and Smad3^-/- mice. Peripheral blood samples were collected from tail veins of mice, and then analyzed on Sysmex F-820. Shown here are the means and standard deviations of total numbers and distributions of blood leukocytes from 4 wild-type and 4 mutant mice (^aP < 0.05, ^bP < 0.01).

Figure 2 Percentage of lymphocyte subpopulations in peripheral blood (A), thymus (B), spleen (C) and lymph nodes (D) of wild-type and mutant mice. The cells were stained with PE-anti-CD4 and FITC-anti-CD8, or with FITC-anti-CD3 and PE-anti-CD19, and then subjected to cytometric analyses. Shown here are the means and standard deviations of percentage of lymphocyte subpopulations from 4 wild-type and 4 mutant mice (^aP < 0.05, ^bP < 0.01).

Figure 3 Percentage of CD4⁺CD25⁺ T cells in peripheral lymphoid tissues of Smad3^+/+ and Smad3^-/- mice. PBMC (blood) or single-cell suspensions from thymus (T), spleen (S), and lymph nodes (L) were prepared according to “MATERIALS AND METHODS”, co-labeled with PE-anti-CD4 and FITC-anti-CD25, and then analyzed by FACScan.