Epstein-Barr virus-associated gastric carcinoma: Evidence of age-dependence among a Mexican population

Figure 1 Moderately differentiated, intestinal-type (tub2) adenocarcinoma. Irregular neoplastic tubular structures are seen throughout the field (hematoxylin and eosin stain).

Figure 2 Same case as in Figure 1. EBER-1 nuclear positivity is limited to neoplastic cells lining the tubular structures (in situ hybridization).

Figure 3 Diffuse-type (por1) adenocarcinoma. Sheets of neoplastic cells are distributed in an indistinct pattern (hematoxylin and eosin stain).

Figure 4 Same case as in Figure 3. A uniform nuclear signal of EBER-1 is seen in neoplastic cells (in situ hybridization)

Figure 5 Poorly differentiated, LEL carcinoma. Clusters of neoplastic cells are separated by lymphoplasmacytic infiltrate

Figure 6 Same case as in Figure 5. An EBER-1-positive signal is detected in the nuclei of neoplastic cells (in situ hybridization

Figure 7 Lining gastric epithelium shows regenerative changes characterized by nuclear growth without atypia. There are few neoplastic cells in the underlying lamina propria (hematoxylin and eosin stain)

Figure 8 Same case as in Figure 7. The EBER-1-positive nuclear signal is restricted to regenerative epithelium. Note the EBER-1 nuclear negativity of neoplastic cells infiltrating the lamina propria (in situ hybridization

Figure 9 Lining gastric epithelium shows high-grade dysplasia, characterized by cell stratification and crowding, increased nuclear/cytoplasm ratio, nuclear atypia, and prominent eosinophilic nucleoli (hematoxylin and eosin stain).

Figure 10 Same case as in Figure 9. Dysplastic epithelium is intensely positive for the EBER-1 nuclear signal (in situ hybridization)