Combined effects of Cantide and chemotherapeutic drugs on inhibition of tumor cells’ growth in vitro and in vivo

Figure 1 HepG2 cells’ growth inhibited by Cantide, DDP(A), 5-FU(B) and ADM(C) only and at the indicated combinations on HepG2 cells. The dots represent the concentrations of chemotherapeutic drugs as 0 on the dose-response curves which means treatments with Cantide only. The cells were treated with Cantide complexed to Lipofectin for 6 h at 37 °C. The medium was then replaced with media containing various concentrations of DDP, 5-FU and ADM. After 72 h of incubation, MTT assay was performed. Absorbance at 490 nm was normalized to the control, untreated cells to determine cell viability. Each value represents the mean±SD from triplicate determination.

Figure 2 Q values for the combination treatments of Cantide with DDP (A), 5-FU (B) and ADM (C) tested on HepG2 cells. Q values were calculated from the dose-response curves shown in Figure 1 and analyzed with Zheng-Jun Jin method.

Figure 3 Tumor growth curves for Balb/c nude mice after treatment with DDP, Cantide or DDP+Cantide. Points for these groups represent the average values for 7-8 mice.