Basic Research
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Apr 28, 2005; 11(16): 2431-2437
Published online Apr 28, 2005. doi: 10.3748/wjg.v11.i16.2431
Figure 1
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Figure 1 The dose-response curves of effect of NCTD on GBC-SD cells for 72 h. Inhibition of the growth of human gallbladder carcinoma GBC-SD cells by various concentrations of NCTD. Cell number was counted by the MTT method.
Figure 2
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Figure 2 The inhibitory effect curves of IC50 NCTD on GBC-SD cells at different times. Cell number was counted by the MTT method.
Figure 3
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Figure 3 Positive expression occurred in cell nucleoli, with brown or yellow dye, of PCNA protein of GBC-SD cells (immunohistochemistry SABC method, ×200). A: The brown dye of PCNA was shown positively in most cells of the control group. B: In the experiment group with treatment of IC50 NCTD for 48 h, the positive cells of PCNA expression decreased significantly and the dye in cell nucleoli became light and shallow.
Figure 4
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Figure 4 The positive expression occurred in cell nucleoli, with brown or yellow dye, of Ki-67 protein of GBC-SD cells (immunohistochemistry SABC method, ×100). A: The brown dye of Ki-67 was shown positively in most cells of the control group. B: In the experiment group with treatment of IC50 NCTD for 48 h, the positive cells of Ki-67 expression decreased significantly and the dye in cell nucleoli became light and shallow.
Figure 5
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Figure 5 Positive expression with brown dye occurred in cytoplast of MMP2 protein of GBC-SD cells (immunohistochemistry SABC method, ×100). A: The brown dye of MMP2 was shown positively in most cells of the control group. B: In the experiment group with treatment of NCTD (5 μg/mL) for 48 h, the positive cells of MMP2 expression decreased significantly and the dye in the cytoplast became light.
Figure 6
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Figure 6 Positive expression with brown dye occurred on nucleoli membrane of TIMP2 protein of GBC-SD cells (immunohistochemistry SABC method, ×100). A: The negative expression of TIMP2 was observed in most GBC-SD cells of the control group. B: In the experiment group with treatment of NCTD (5 μg/mL) for 48 h, the positive cells of TIMP2 expression were increased significantly and the dye on nucleoli membrane represent brown.