c-src activating mutation analysis in Chinese patients with colorectal cancer

Figure 1 PCR amplification of src exon 12 from genomic DNA of colorectal cancer. Lanes 1-8: samples; lane M: marker.

Figure 2 Positive result of No. 73 sample in RFLP analysis. Lane 1: No. 73 para-cancer tissue; lane 2: No. 73 cancer tissue; lane 3: positive control; lane M: DNA marker.

Figure 3 Sequencing of two clones (one wild and the other Amber-mutated) from RFLP-positive PCR products of No. 73 colorectal cancer.

Figure 4 SSCP analysis of src exon 12 fragment amplified from colorectal cancer. Lane 1: positive control; lane 2: No. 73; lane 3: negative control; lanes 4-6: three negative samples.