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©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 21, 2005; 11(15): 2351-2353
Published online Apr 21, 2005. doi: 10.3748/wjg.v11.i15.2351
Published online Apr 21, 2005. doi: 10.3748/wjg.v11.i15.2351
c-src activating mutation analysis in Chinese patients with colorectal cancer
Ye-Xiong Tan, Peng Zhang, Zhong-Hua Yan, Meng-Chao Wu, Hong-Yang Wang, International Co-operational Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China
Han-Tao Wang, Guan-Long Dai, Department of General Surgery, Changhai Hospital, Shanghai 200433, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Hong-Yang Wang, International Co-operational Laboratory on Signal Transduction, Eastern Hepatobiliary Institute, Shanghai 200438, China. hywangk@online.sh.cn
Telephone: +86-21-25070856 Fax: +86-21-65566851
Received: April 24, 2004
Revised: April 25, 2004
Accepted: April 29, 2004
Published online: April 21, 2005
Revised: April 25, 2004
Accepted: April 29, 2004
Published online: April 21, 2005
Abstract
AIM: To investigate the occurrence of cellular src (c-src) activating mutation at codon 531 in colorectal cancer patients from Chinese mainland.
METHODS: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay followed by sequencing and single-strand conformation polymor-phism analysis were carried out to screen 110 samples of primary colorectal cancer and 20 colorectal liver metastases.
RESULTS: Only one sample showed PCR-RFLP-positive results and carried somatic codon 531 mutations. No additional mutation of c-src exon 12 was found.
CONCLUSION: c-src codon 531 mutation in colorectal cancer is not the cause of c-src activation.
Keywords: c-src; Colorectal cancer