In vitro growth inhibition of human colonic tumor cells by Verapamil

Figure 1 Inhibition of proliferation of HCT cells by Verapamil. Results were expressed as mean±SD n = 9 ^bP<0.01 vs control.

Figure 2 The percentage of S phase HCT cells treated with Verapamil. With increase in Verapamil concentration, the percent of S phase cells was decreased as detected by flow cytometry.

Figure 3 Flow cytometry detection of HCT cells apoptosis. The percentage of apoptotic cells increased as Verapamil concentration increased. A: control; B: 1 μmol/L Verapamil; C: 10 μmol/L Verapamil; D: 100 μmol/L Verapamil; E: 500 μmol/L Verapamil.

Figure 4 DNA Ladder of HCT cell line treated with Verapamil. M: marker (100 bp DNA Ladder); Con: control; Vp1: 100 μmol/L Verapamil; Vp2: 200 μmol/L Verapamil; Vp3: 400 μmol/L Verapamil.

Figure 5 Cellular morphologic changes (400×). Cell membrane blebs and the cytoplasm and nuclear chromatin condensation in HCT cells treated with Verapamil were observed. A: control cells; B: 100 μmol/L Verapamil.

Figure 6 Nuclear model change as reniform and nuclear chromatin condensation in HCT cell line treated with Verapamil and labeled with PI (400×). A: Control cells; B: 100 μmol/L Verapamil.

Figure 7 Influence of Verapamil on [Ca²⁺]_i intensity in HCT cell after 12 h (400×). 100 μmol/L. A: Control cells; B: 100 μmol/L Verapamil.