Effects of glutamine supplementation on splenocyte cytokine mRNA expression in rats with septic peritonitis

Figure 1 Expression of interleukin (IL)-2, IL-4, IL-10, interferon (IFN)-γ and tumor necrosis factor (TNF)-α messenger RNA in the spleen as determined by RT-PCR (control, n = 9; group 1, n = 11; group 2, n = 9; group 3, n = 9; group 4, n = 11). A: Expression of IL-2 messenger RNA in the spleen. ^aP<0.05 vs control group and group 1 (-/-); B: Expression of IL-4 messenger RNA in the spleen. ^cP<0.05 vs control group and group 1 (-/-); C: Expression of IL-10 messenger RNA in the spleen. ^eP<0.05 vs control group, group 1 (-/-) and group 3 (+/-); ^gP<0.05 vs control group and group 1 (-/-); D: Expression of IFN-γ messenger RNA in the spleen. ⁱP<0.05 vs experimental groups 2, 3, 4; E: Expression of TNF-α messenger RNA in the spleen. ^kP<0.05 vs group 2 (-/+), group 3 (+/-) and group 4 (+/+).

Figure 2 Distributions of CD45Ra⁺, CD3⁺, CD4⁺, and CD8⁺ in splenocytes from control group and experimental groups 3 d after CLP. ^aP<0.05 vs the other groups; ^cP<0.05 vs control group.