Basic Research
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Mar 28, 2005; 11(12): 1742-1746
Published online Mar 28, 2005. doi: 10.3748/wjg.v11.i12.1742
Effects of glutamine supplementation on splenocyte cytokine mRNA expression in rats with septic peritonitis
Sung-Ling Yeh, Yu-Ni Lai, Huey-Fang Shang, Ming-Tsan Lin, Wan-Chun Chiu, Wei-Jao Chen
Sung-Ling Yeh, Yu-Ni Lai, Wan-Chun Chiu, Institute of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan, China
Huey-Fang Shang, Department of Microbiology and Immunology, Taipei Medical University, Taipei, Taiwan, China
Ming-Tsan Lin, Wei-Jao Chen, Department of Surgery, College of Medicine, National Taiwan University, Taipei, Taiwan, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Wei-Jao Chen, M.D., Department of Surgery, National Taiwan University Hospital, 7 Chung-Shan S. Road, 100 Taipei, Taiwan, China. chenwj@ccms.ntu.edu.tw
Telephone: +886-62-23970800-2122 Fax: +886-62-23412969
Received: March 9, 2004
Revised: March 10, 2004
Accepted: May 13, 2004
Published online: March 28, 2005
Abstract

AIM: To investigate the effects of glutamine (GLN)-enriched diets before and GLN-containing total parenteral nutrition (TPN) after sepsis or both on the secretion of cytokines and their mRNA expression levels in splenocytes of rats with septic peritonitis.

METHODS: Rats were assigned to a control group and 4 experimental groups. The control group and experimental groups 1 and 2 were fed a semipurified diet, while experimental groups 3 and 4 had part of the casein replaced by GLN which provided 25% of the total nitrogen. After rats were fed with these diets for 10 d, sepsis was induced by cecal ligation and puncture (CLP), whereas the control group underwent a sham operation, at the same time, an internal jugular vein was cannulated. All rats were maintained on TPN for 3 d. The control group and experimental groups 1 and 3 were infused with conventional TPN, while the TPN in experimental groups 2 and 4 was supplemented with GLN, providing 25% of the total nitrogen in the TPN solution. All rats were kiued 3 d after sham operation or CLP to examine their splenocyte subpopulation distribution and cytokine expression levels.

RESULTS: Most cytokines could not be detected in plasma except for IL-10. No difference in plasma IL-10 was observed among the 5 groups. The IL-2, IL-4, IL-10, and TNF-α mRNA expression levels in splenocytes were significantly higher in experimental groups 2 and 4 than in the control group and group 1. The mRNA expression of IFN-γ was significantly higher in the GLN-supplemented groups than in the control group and experimental group 1. The proportion of CD45Ra+ was increased, while those of CD3+ and CD4+ were decreased in experimental group 1 after CLP was performed. There were no differences in spleen CD3+ lymphocyte distributions between the control and GLN-supplemented groups.

CONCLUSION: GLN supplementation can maintain T-lymphocyte populations in the spleen and significantly enhance the mRNA expression levels of Th1 and Th2 cytokines and TNF-α in the spleen of rats with septic peritonitis.

Keywords: Glutamine; Total parenteral nutrition