Transfection of p27kip1 enhances radiosensitivity induced by 60Co γ-irradiation in hepatocellular carcinoma HepG2 cell line

Figure 1 Effect of irradiation on proliferation of HepG₂ cells.

Figure 2 Counterstaining of Hoechst 33258 and propidium iodide to distinguish apoptotic from necrotic cells.

Figure 3 Comparsion of apoptosis and necrosis rate of HepG₂ treated by ⁶⁰Co γ-irradiation. ^aP < 0.05, ^bP < 0.01 vs necrosis.

Figure 4 Subcellular localization and expression of p27^kip1 in tranfected HepG₂ cells.

Figure 5 Flow cytometry analysis of cell cycle of nontransfected and transiently transfected HepG₂ cells. A: Nontransfected HepG₂ cells. B: p27^kip1 transiently transfected HepG₂ cells.

Figure 6 Apoptotic rates induced by γ-irradiation in nontransfected and transfected HepG₂ cells. ^bP < 0.01 vs control.