Prolongation of liver allograft survival by dendritic cells modified with NF-κB decoy oligodeoxynucleotides

Figure 1 Inhibition of IL-4 or LPS -induced NF-κB activation in DCs by NF-κB decoy ODNs. Nuclear proteins of GM-CSF DCs, GM-CSF + IL-4 DCs, GM-CSF+IL-4 + NF-κB decoy ODNs DCs, GM-CSF+IL-4 + scrambled ODNs DCs, GM-CSF + LPS DCs, GM-CSF + LPS+ NF-κB decoy ODNs DCs and GM-CSF + LPS + scrambled ODNs DCs were measured by EMSA (lanes 1-7).

Figure 2 Suppression of IL-4-induced costimulatory molecule expression in DCs by NF-κB decoy ODNs.

Figure 3 Suppression of IL-12 protein expression in LPS-stimulated DCs by NF-κB decoy ODNs. Protein extracts from GM-CSF DCs, GM-CSF + LPS DCs, GM-CSF + LPS + NF-κB decoy ODNs DCs and GM-CSF + LPS +scrambled ODNs DCs were measured by Western blot (lane 1-4).

Figure 4 Suppression of allostimulatory function of IL-4-stimu-lated DCs by NF-κB decoy ODNs. ^bP < 0.001 vs IL-4 + NF-κB decoy ODNs -propagated DCs.

Figure 5 Prolongation of survival of liver allograft recipient by NF-κB decoy ODNs-treated DCs. ^bP < 0.001 vs group A, ^dP < 0.001 vs group A, ^fP < 0.001 vs group C, ^hP < 0.001 vs group A, ^jP < 0.001 vs group B, ^lP < 0.001 vs group B, ^aP > 0.05 vs group B.

Figure 6 Augmentation of apoptosis of liver allograft-infiltrat-ing cells in portals. by NF-κB decoy ODNs-treated DCs ( × 400).

Figure 7 Suppression of IL-2 and IFN-γ mRNA expression and up-regulation of IL-4 mRNA expression both in liver graft and in recipient spleen by NF-κB decoy ODNs-modified DCs.